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Analysis of 3-morpholinosydnonimine and sodium nitroprusside effects on dopamine release in the striatum of freely moving rats: role of nitric oxide, iron and ascorbic acid

Serra, Pier Andrea and Esposito, Giovanni and Delogu, Maria Rosaria and Migheli, Rossana and Rocchitta, Gaia Giovanna Maria and Grella, Giuseppe Enrico and Miele, Egidio and Miele, Maddalena and Desole, Maria Speranza (2000) Analysis of 3-morpholinosydnonimine and sodium nitroprusside effects on dopamine release in the striatum of freely moving rats: role of nitric oxide, iron and ascorbic acid. British Journal of Pharmacology, Vol. 131 (4), p. 836-842. eISSN 1476-5381. Article.

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DOI: 10.1038/sj.bjp.0703635

Abstract

1 The effects of intrastriatal infusion of 3-morpholinosydnonimine (SIN-1) or sodium nitroprusside (SNP) on dopamine (DA), 3-methoxytyramine (3-MT), dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), L-dihydroxyphenylalanine (L-DOPA), ascorbic acid and uric acid concentrations in dialysates from the striatum of freely moving rats were evaluated using microdialysis. 2 SIN-1 (1 mM) infusion for 180 min increased microdialysate DA and 3-MT concentrations, while L-DOPA, DOPCA+HVA, ascorbic acid and uric acid levels were unaffected. Co-infusion with ascorbic acid (0.1 mM) inhibited SIN-1-induced increases in DA and 3-MT dialysate concentration. 3 SNP (1 mM) infusion for 180 min increased greatly the dialysate DA concentration to a peak (2950% of baseline) at the end of the infusion, while increases in 3-MT were negligible. In addition, SNP decreased ascorbic acid and L-DOPA but increased uric acid concentration in the dialysate. Co-infusion with deferoxamine (0.2 mM) inhibited the late SNP-induced increase in DA dialysate concentration, but did not affect the decrease in ascorbic acid and increase uric acid dialysate concentrations. 4 SNP (1 mM) infusion for 20 min moderately increased uric acid, DA and 3-MT, but decreased L- DOPA levels in the dialysate. Ascorbic acid concentration increased at the end of SNP infusion. Co- infusion with ascorbic acid (0.1 mM) inhibited the SNP-induced increase in DA and 3-MT, but did not affect the decrease in L-DOPA and increase in uric acid dialysate concentrations. 5 These results suggest that NO released from SIN-1 may account for the increase in the dialysate DA concentration. NO released following decomposition of SNP may account for the early increase in dialysate DA, while late changes in microdialysate composition following SNP may result from an interaction between NO and the ferrocyanide moiety of SNP. Exogenous ascorbic acid inhibits the effect of exogenous NO on DA release probably by scavenging NO, suggesting that endogenous ascorbic acid may modulate the NO control of DA release from 300 striatal dopaminergic terminals.

Item Type:Article
ID Code:610
Status:Published
Refereed:Yes
Uncontrolled Keywords:NO-donor, dopamine, in vivo release, ascorbic acid, iron, microdialysis, rat striatum
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 03 - Scienze chimiche > CHIM/08 Chimica farmaceutica
Divisions:001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
001 Università di Sassari > 01 Dipartimenti > Farmaco, chimico, tossicologico
Publisher:Macmillan
eISSN:1476-5381
Deposited On:18 Aug 2009 10:02

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