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Multilocus microsatellite polymorphism analysis to characterize Leishmania infantum strains isolated in Sicily

Reale, Stefano and Lupo, T. and Migliazzo, Aldo and Dimauro, Corrado and Ciprì, V. and Calderone, Simona and Manna, Laura and Vitale, Fabrizio (2010) Multilocus microsatellite polymorphism analysis to characterize Leishmania infantum strains isolated in Sicily. Transboundary and Emerging Diseases, Vol. 57 (1-2), p. 37-41. eISSN 1865-1682. Article.

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DOI: 10.1111/j.1865-1682.2010.01131.x


Different approaches are being developed to improve the differentiation of Leishmania genus using biochemical and molecular methods. In this study, 11 independent polymorphic microsatellites were used for the typing of strains of L. infantum isolated in Sicily. Polymerase chain reaction was employed to amplify the microsatellites contained in 12 DNA regions selected from among more investigated loci. A total of 51 isolates of L. infantum from dogs were tested by using the same locus panel. The products were successively analysed using an automatic sequence detector (ABI PRISM 3130 AB), to discover relevant microsatellite polymorphisms. It was possible to discriminate between MON-1 and non-MON-1 groups. Moreover, the method permitted to distinguish various genotypes of L. infantum isolates within each zymodema. Modeland distance-based analyses of the data set showed comparable results. The frequency of heterozygosity in the alleles analysed varied extremely between the different groups of isolates. As the method exhibits a high level of discrimination, it is suitable for characterization of closely related strains in epidemiological studies.

Item Type:Article
ID Code:5972
Uncontrolled Keywords:Leishmania, zymodema, polymerase chain reaction, microsatellites, multilocus enzyme, electrophoresis, multilocus microsatellite typing
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
Publisher:Blackwell / Wiley
Additional Information:Special issue: One health, one medicine: tackling the challenge of emerging diseases
Deposited On:20 Apr 2011 09:47

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