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Adenovirus-mediated human tissue kallikrein gene delivery inhibits neointima formation induced by interruption of blood flow in mice

Emanueli, Costanza and Salis, Maria Bonaria and Chao, Julie and Chao, Lee and Agata, Jun and Lin, Kuei-Fu and Munaò, Antonella and Straino, Stefania and Minasi, Alessandra and Capogrossi, Maurizio C. and Madeddu, Paolo Roberto (2000) Adenovirus-mediated human tissue kallikrein gene delivery inhibits neointima formation induced by interruption of blood flow in mice. Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 20 (6), p. 1459-1466. eISSN 1524-4636. Article.

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Abstract

Tissue kallikrein cleaves kininogen to produce vasoactive kinin peptides. Binding of kinins to bradykinin B2 receptors on vascular endothelial cells stimulates the release of nitric oxide and prostacyclin, thus activating the cGMP and cAMP pathways. In this study, we evaluated the effects of adenovirus-mediated human tissue kallikrein gene (Ad.CMV-cHK) delivery in a mouse model of arterial remodeling induced by permanent alteration in shear stress conditions. Mice underwent ligature of the left common carotid artery and were injected intravenously with saline or 1.8x109 plaque-forming units of Ad.CMV-cHK or control virus (Ad.CMV-LacZ). Fourteen days after surgery, morphometric analysis revealed that Ad.CMV-cHK reduced neointima formation by 52% (P<0.05) compared with Ad.CMV-LacZ. Expression of human tissue kallikrein (HK) mRNA was detected in mouse carotid artery, aorta, kidney, heart, and liver, and recombinant HK was present in the urine and plasma of mice receiving HK gene. Kallikrein gene transfer resulted in increases in urinary kinin, cGMP, and cAMP levels. The protective action of Ad.CMV-cHK on neointima formation was significantly reduced (P<0.05) in mice with knockout of the kinin B2 receptor gene compared with wild-type control mice (J129Sv mice). In contrast, the effect of Ad.CMV-cHK was amplified (P<0.05) in transgenic mice overexpressing human B2 receptor compared with wild-type control mice (c57/Bl6 mice). Thus, the inhibitory effect of recombinant kallikrein on structural alterations caused by the interruption of blood flow appears to be mediated by the B2 receptor. These results provide new insight into the role of the tissue kallikrein-kinin system in vascular remodeling and suggest the application of HK gene therapy to treat restenosis and atherosclerosis.

Item Type:Article
ID Code:594
Status:Published
Refereed:Yes
Uncontrolled Keywords:Human tissue kallikrein, gene delivery, neointima formation, bradykinin B2 receptors, transgenic mice, knockout
Subjects:Area 06 - Scienze mediche > MED/09 Medicina interna
Divisions:001 Università di Sassari > 03 Istituti > Clinica medica generale e terapia medica
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo > Cardiovascular medicine and gene therapy Section
Publisher:Lippincott Williams & Wilkins
eISSN:1524-4636
Copyright Holders:© American Heart Association
Deposited On:18 Aug 2009 10:02

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