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αS1-casein in goat milk: identification of genetic variants by Capillary Zone Electrophoresis compared to Isoelectric Focusing

Brambilla, A. and Feligini, Maria and Enne, Giuseppe (2003) αS1-casein in goat milk: identification of genetic variants by Capillary Zone Electrophoresis compared to Isoelectric Focusing. Italian Journal of Animal Science, Vol. 2 (Suppl. 1), p. 100-102. eISSN 1828-051X. Article.

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Abstract

AlphaS1 casein fraction in caprine milk is characterized by an important polymorphism due to substitution, deletion of amino acids and post trascriptional modifications (Grosclaude et al., 1994; Ferranti et al., 1997). This structural polymorphism is associated to a quantitative variability in protein expression related to different milk quality and dairy properties (Pierre et al., 1998; Remeuf, 1993; Vassal et al., 1994). Classical electrophoretic methods were applied to characterize the phenotypic variants at αS1-casein fraction (Addeo et al., 1988; Russo et al., 1986). During the last ten years capillary electrophoresis became an analytical technique for rapid and automated analysis requiring small sample volume and small solvent waste. These characteristics, together with the high resolution and the chance to give quantitative results, made this technique a useful tool in studying milk protein characterization and in detecting adulteration (Cattaneo et al., 1996a; 1996b) in different application fields. CZE was applied to the study of caprine milk proteins to quantify high, medium and low αS1- casein content and to identify genetic variants αS1 A, B and C on the basis of their different migration time (Recio et al., 1997). The aim of this work was to test a CZE procedure able to identify and discriminate the main αS1 caprine variants A, B, E and F through specific and repeatable electromigration patterns. Comparison between CZE and IEF assays is discussed.

Item Type:Article
ID Code:5805
Status:Published
Refereed:Yes
Uncontrolled Keywords:Capra hircus, αS1-casein, capillary zone electrophoresis, isoelectric focusing
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
Publisher:Avenue media on behalf of Scientific Association of Animal Production (ASPA)
eISSN:1828-051X
Publisher Policy:Depositato per gentile concessione dell'ASPA
Additional Information:Relazione presentata al 15. Congresso nazionale ASPA, Parma, 18-20 giugno 2003.
Deposited On:23 Mar 2011 12:32

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