Mulas, Gilberto and Galaffu, Maria Grazia and Pretti, Luca and Nieddu, Giovanni and Mercenaro, Luca and Tonelli, Roberto and Anedda, Roberto (2011) NMR analysis of seven selections of vermentino grape Berry: metabolites composition and development. Journal of Agricultural and Food Chemistry, Vol. 59 (3), p. 793-802. eISSN 1520-5118. Article.
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The goal of this work was to study via NMR the unaltered metabolic profile of Sardinian Vermentino grape berry. Seven selections of Vermentino were harvested from the same vineyard. Berries were stored and extracted following an unbiased extraction protocol. Extracts were analyzed to investigate variability in metabolites concentration as a function of the clone, the position of berries in the bunch or growing area within the vineyard. Quantitative NMR and statistical analysis (PCA, correlation analysis, Anova) of the experimental data point out that, among the investigated sources of variation, the position of the berries within the bunch mainly influences the metabolic profile of berries, while the metabolic profile does not seem to be significantly influenced by growing area and clone. Significant variability of the amino acids such as arginine, proline, and organic acids (malic and citric) characterizes the rapid rearrangements of the metabolic profile in response to environmental stimuli. Finally an application is described on the analysis of metabolite variation throughout the physiological development of berries.
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