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A Stress responsive alternative splicing mechanism in Citrus clementina leaves

Del Carratore, Renata and Magaldi, Eleonora and Podda, Alessandra and Beffy, Pascale Brigitte and Migheli, Quirico and Maserti, Bianca Elena (2011) A Stress responsive alternative splicing mechanism in Citrus clementina leaves. Journal of Plant Physiology, Vol. 168 (9), p. 952-959. eISSN 1618-1328. Article.

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DOI: 10.1016/j.jplph.2010.11.016

Abstract

Chitinases are often considered pathogenesis-related proteins since their activity can be induced by viral infections, fungal and bacterial cell wall components, and also by more general sources of stress such as wounding, salicylic acid, ethylene, auxins and cytokinins. In the present study, comparative proteomic analysis showed the defense-related acidic chitinase II to be specifically induced in Citrus clementina leaves infested by the two-spotted spider mite Tetranychus urticae or treated with MeJA. In parallel, changes in the mRNA profiles of two partially homologous chitinase forms were shown by RT-PCR. In particular, the appearance of an additional cDNA chitinase fragment in T. urticae-infested and MeJA-treated leaves was observed. This finding may indicate a specific regulatory mechanism of chitinase expression. We report evidence for alternative splicing in T. urticae-infested C. clementina, where a premature stop codon after the first 135 amino acids was introduced. We observed inducible chitinase activity after MeJA treatment, indicative of a rapid plant response to infestation. This work provides the first evidence of chitinase alternative splicing in C. clementina. In addition, the presence of the dual-band pattern for chitinase cDNA by RT-PCR may represent a suitable predictive marker for early diagnosis of plant biotic stress.

Item Type:Article
ID Code:5770
Status:Published
Refereed:Yes
Uncontrolled Keywords:Citrus clementina, chitinase, Tetranychus urticae, alternative splicing, methyl jasmonate
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:Urban and Fischer / Elsevier
eISSN:1618-1328
Copyright Holders:© 2011 Elsevier
Deposited On:21 Mar 2011 12:44

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