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Measurement of carnosine, homocarnosine and anserine by FASI capillary electrophoresis UV detection: applications on biological samples

Zinellu, Angelo and Sotgia, Salvatore and Campesi, Ilaria and Franconi, Flavia and Deiana, Luca and Carru, Ciriaco (2011) Measurement of carnosine, homocarnosine and anserine by FASI capillary electrophoresis UV detection: applications on biological samples. Talanta, Vol. 84 (3), p. 931-935. ISSN 0039-9140. Article.

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DOI: 10.1016/j.talanta.2011.02.030

Abstract

A field amplified sample injection (FASI) capillary electrophoresis method with UV detection was developed for the separation and detection of carnosine-related peptides carnosine (Car), anserine (Ans) and homocarnosine (Hcar). The imidazole dipeptides were baseline-separated within 10 min by using 50 mmol/L Tris phosphate pH 2.2 as running buffer. The samples were diluted in water and directly injected on the capillary without complex cleanup and/or sample derivatization procedures. Using the electrokinetic injection, a sensitivity improvement of about 500-fold was achieved without any loss of separation efficiency if compared to the conventional sample injection. The detection limits for carnosine, anserine, and homocarnosine were between 0.4 and 0.5 nmol/L, thus improving of 10- to 100-fold the LOD of previous described methods based on laser induced fluorescence or chemiluminescence detection. This method has been applied to the analysis of homogenized rat tissue (heart, muscle and brain) and human cerebrospinal fluid (CSF).

Item Type:Article
ID Code:5752
Status:Published
Refereed:Yes
Uncontrolled Keywords:Anserine, carnosine, homocarnosine, FASI, capillary electrophoresis
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo
Publisher:Elsevier
ISSN:0039-9140
Deposited On:11 May 2011 10:59

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