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Regulation of human endogenous retroviruses of the W family by type III interferon λ2 and HIV in astrocytes

Mei, Alessandra and Mameli, Giuseppe and Serra, Caterina and Poddighe, Luciana and Uleriand, Elena and Dolei, Antonina (2009) Regulation of human endogenous retroviruses of the W family by type III interferon λ2 and HIV in astrocytes. Cytokine, Vol. 48 (1-2), p. 65. eISSN 1096-0023. Article.

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DOI: 10.1016/j.cyto.2009.07.209

Abstract

Astrocytes play a key role in important neurological diseases such as multiple sclerosis (MS) and neuroAIDS. The multiple sclerosis-associated retrovirus (MSRV) is an active member of the W family of human endogenous retroviruses (HERV), able to produce extracellular particles; another HERV-W element is ERVWE1, producing only an env protein, named syncytin-1, that can be found intracellularly and on the plasma-membrane, but not extracellularly. MSRVenv and syncytin-1 proteins have pro-inflammatory properties that might be pathogenic, as observed in vitro and in animal models: they may cause neuroinflammation, neurodegeneration, alterations of the immune system and stress responses; both have been suggested as co-factors triggering the immunopathogenesis of MS.λ2 has been shown to favour the replication of exogenous retroviruses, such as HIV, we designed studies to detect possible effects of IFNλ2 on the above HERV-W retroelements in U87 MG astrocytes, by using real time RT-PCR assays that can selectively identify either MSRVenv or syncytin-1 transcripts.. Treatment of astrocytes with IFNλ2 was found to enhance the expression of both MSRVenv and syncytin-1, dose-dependently. If cells are infected by HIV, the expression of both elements is modified. Surprisingly, HIV caused opposite effects on the two HERV-W retroelements, since MSRVenv transcription was up-regulated, whilst that of syncytin-1 was down-regulated. Cell pre-treatment with IFNλ2 did not alter the effects of subsequent HIV infection on MSRVenv and syncytin-1. behaves as a pro-inflammatory cytokine, that can facilitate the expression of both exogenous and endogenous human retroviruses.
Since Type III IFN.
This cell line has the IL-28R receptor, and is sensitive to IFNλ2.
It is totally unknown the role played by Type III IFN in neuropathologies like neuro-AIDS and MS, but it could be hypothesized that IFNλ.

Item Type:Article
ID Code:5722
Status:Published
Refereed:Yes
Uncontrolled Keywords:Astrocytes, multiple sclerosis-associated retrovirus (MSRV), human endogenous retroviruses (HERV), HIV
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Academic Press / Elsevier
eISSN:1096-0023
Copyright Holders:© 2009 Published by Elsevier
Additional Information:Poster presented at: Tri-Society annual conference 2009 of the Society for leukocyte biology, international cytokine society, & International society for interferon and cytokine research on Cellular and cytokine interactions in health and disease, Lisbon, Portugal, October 18-21, 2009.
Deposited On:03 May 2011 13:37

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