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Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cells

Bonelli, Piero and Nicolussi, Paola and Manetti, Roberto and Antuofermo, Elisabetta and Dattena, Maria (2010) Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cells. Italian Journal of Animal Science, Vol. 9 (1), p. 26-28. eISSN 1828-051X. Article.

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DOI: 10.4081/ijas.2010.e6

Abstract

CD34 is a cell surface glycoprotein expressed by hematopoietic progenitors and endothelial cells. It is widely used in the clinic for isolation of human hematopoietic stem cells. In recent years large animals are gaining increasing importance in biomedical research for the study and therapy of human diseases. Sheep has proved to be an useful experimental model for preclinical trials in transplantation procedures. Unfortunately, the lack of specie-specific monoclonal antibodies (MABS) recognizing hemopoietic progenitor cells hampers the use of this animal in experimental hematology. The aim of this paper was to determine whether commercial monoclonal antibodies specific for human CD34 molecule could cross-react with hematopoietic progenitor cells (HPC) present in sheep umbilical cord blood (UCB). Six anti-human CD34 MABS, recognizing the three different epitope classes, were tested in flow cytometry on purified mononuclear cells (MNC) isolated from cord blood of both species. None of the MABS used in this trial seemed to be able to identify HPC from sheep UCB. These data suggest that the panel of monoclonal antibodies used for cross reactivity detection has to be expanded with recently produced reagents. Further studies should be directed towards the production of ovine specific anti CD34 MABS.

Item Type:Article
ID Code:5721
Status:Published
Refereed:Yes
Uncontrolled Keywords:CD 34, hemopoietic progenitor cells, cross-reactivity, sheep
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/03 Patologia generale e anatomia patologica veterinaria
Area 06 - Scienze mediche > MED/09 Medicina interna
Divisions:001 Università di Sassari > 03 Istituti > Clinica medica generale e terapia medica
002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zooprofilattico sperimentale della Sardegna, Sassari
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
Publisher:PAGEPress
eISSN:1828-051X
Copyright Holders:© P. Bonelli et al., 2010 Licensee PAGEPress, Italy
Deposited On:07 Mar 2011 12:00

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