UnissResearch

Logo Universitàegli studi di Sassari
titoli, abstracts, parole chiave >>>
Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay

Penna, Antonella and Battocchi, Cecilia and Garcés, Esther and Anglès, Silvia and Cucchiari, Emellina and Totti, Cecilia and Kremp, Anke and Satta, Cecilia Teodora and Giacobbe, Maria Grazia and Bravo, Isabel and Bastianini, Mauro (2010) Detection of microalgal resting cysts in European coastal sediments using a PCR-based assay. Deep Sea Research. Part II: Topical Studies in Oceanography, Vol. 57 (3-4), p. 288-300. ISSN 0967-0645. Article.

Full text not available from this repository.

DOI: 10.1016/j.dsr2.2009.09.010

Abstract

A PCR-based assay was developed and applied to sediment and sediment trap samples for the detection of different cysts belonging to dinoflagellates and raphidophytes in European coastal areas. Oligonucleotide primers were designed based on the ITS-5.8S and LSU ribosomal gene sequences. The specificity and sensitivity of the PCR assay were assessed using genomic DNA from clonal cultures, plasmid copy number of cloned target sequences, as well as from sediment samples. Qualitative PCR determinations of different cysts in sediment and sediment trap samples were compared to taxonomic examinations by light microscopy. This molecular methodology permitted a fast and specific detection of target cysts in sediment samples. We also detected dinoflagellate and raphidophyte cysts at concentrations not detectable by microscopic methods or that are difficult to identify. The results given by molecular and microscopic methods were comparable. However, higher values of positive detection for target cysts were obtained by PCR than with microscopy. Some taxa were detected in 100% of the samples using PCR, while others were only found in 10% of the samples. The data obtained in this study showed that the PCR-based method is a valid tool for cyst identification in marine sediments.

Item Type:Article
ID Code:5720
Status:Published
Refereed:Yes
Uncontrolled Keywords:HAB species, Mediterranean Sea, PCR, ribosomal genes, resting stages, sediments
Subjects:Area 05 - Scienze biologiche > BIO/07 Ecologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze botaniche, ecologiche e geologiche
Publisher:Pergamon / Elsevier
ISSN:0967-0645
Copyright Holders:© 2009 Elsevier
Additional Information:Title of issue: Phytoplankton life-cycles and their impacts on the ecology of harmful algal bloom
Deposited On:07 Mar 2011 17:08

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page