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Use of random amplified polymorphic DNA (RAPD) to identify races 1, 2, 4 and 8 of Fusarium oxysporum f. sp. dianthi in Italy

Migheli, Quirico and Briatore, Elena and Garibaldi, Angelo (1998) Use of random amplified polymorphic DNA (RAPD) to identify races 1, 2, 4 and 8 of Fusarium oxysporum f. sp. dianthi in Italy. European Journal of Plant Pathology, Vol. 104 (1), p. 49-57. eISSN 1573-8469. Article.

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DOI: 10.1023/A:1008682328764

Abstract

The RAPD fingerprinting procedure was used in combination with pathogenicity assays on differential cultivars to characterize a representative collection of 72 Fusarium spp. isolates of different geographic origin collected from diseased carnation. In F. oxysporum f. sp.dianthi, isolates were grouped according to the physiologic race: group 1 included isolates of race 4; group 2 was formed by isolates of race 2 and single representatives of races 5 and 6; group 3 included isolates of races 1 and 8. No correlation was found between RAPD data and geographic origin of the isolates tested: representatives of race 2 isolated in Italy, Israel and Japan had the same amplification profile. Three isolates which showed a low level of pathogenicity on all carnation cultivars tested shared an identical amplification pattern and are probably saprophytic F. oxysporum. Finally, two F. redolens isolates from Japan and seven nonpathogenic isolates of F. proliferatum collected from diseased carnation in Italy, Israel and The Netherlands were clearly distinguishable according to their RAPD fingerprint. The results are discussed in relation to previous studies on the genetic diversity of F. oxysporum f. sp. dianthi and to the development of forma specialisand pathotypespecific diagnostic tools.

Item Type:Article
ID Code:561
Status:Published
Refereed:Yes
Uncontrolled Keywords:Fusarium spp., Dianthus caryophyllus, physiological races, RAPD-PCR, diagnostics
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:Springer Netherlands
eISSN:1573-8469
Copyright Holders:© 1998 Kluwer Academic Publishers
Deposited On:18 Aug 2009 10:02

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