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Development of PCR primers for a new Fusarium oxysporum pathogenic on Paris daisy (Argyranthemum frutescens L.)

Pasquali, Matias and Acquadro, Alberto and Balmas, Virgilio and Migheli, Quirico and Gullino, Maria Lodovica and Garibaldi, Angelo (2004) Development of PCR primers for a new Fusarium oxysporum pathogenic on Paris daisy (Argyranthemum frutescens L.). European Journal of Plant Pathology, Vol. 110 (1), p. 7-11. eISSN 1573-8469. Article.

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DOI: 10.1023/B:EJPP.0000010141.37327.d0

Abstract

The inverse PCR technique was applied to clone genomic DNA flanking insertion sites of sequences homologous to the transposable element Fot1 in the genome of a new pathogenic isolate of Fusarium oxysporum obtained from wilted Argyranthemum frutescens (Paris daisy). Based on the genomic flanking regions, a primer was designed which when paired to a second primer matching the Fot1 sequence allowed detection of this pathogen by PCR. The primer pair Mg5/Mg6 could specifically identify nine tested isolates of F. oxysporum from A. frutescens, when fungal genomic DNA was used as template. Moreover, the primer pair Mg5/Mg6 allowed successful detection of the pathogen in stem and root tissue from asymptomatic plants that were artificially inoculated with a representative isolate of F. oxysporum from A. frutescens.

Item Type:Article
ID Code:559
Status:Published
Refereed:Yes
Uncontrolled Keywords:Transposons, Fot1, inverse PCR, molecular diagnosis
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:Kluwer Academic
eISSN:1573-8469
Deposited On:18 Aug 2009 10:02

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