UnissResearch

Logo Universitàegli studi di Sassari
titoli, abstracts, parole chiave >>>
Differential distribution of structural components and hydration in aortic and pulmonary heart valve conduits: impact of detergent-based cell removal

Naso, Filippo and Gandaglia, Alessandro and Formato, Marilena and Cigliano, Antonio and Lepedda, Antonio Junior and Gerosa, Gino and Spina, Michel (2010) Differential distribution of structural components and hydration in aortic and pulmonary heart valve conduits: impact of detergent-based cell removal. Acta Biomaterialia, Vol. 6 (12), p. 4675-4688. eISSN 1878-7568. Article.

Full text not available from this repository.

DOI: 10.1016/j.actbio.2010.06.037

Abstract

Evaluation of the physiological performance of biological scaffolds for tissue engineering applications has been mostly based on biophysical and morphological methods, with limited attention paid to the quantitative contribution of the main structural components to native and/or treated valve assemblies. In the present study quantitation addressed the porcine leaflet, sinus and adjacent wall of aortic and pulmonary valved conduits before and after detergent-based cell removal. Collagen, elastin, glycosaminoglycan, lipid and water contents were expressed in terms of relative concentration and volume fraction in order to assess their effective contribution to the native tissue and to changes following decellularization procedures. The main findings were recognition of unexpectedly large water and underestimated collagen contents, differential distribution of elastin between the sectors and of glycosaminoglycan along the conduits and pulmonary scaffold destabilization upon cell removal, not found in the aortic case. Simultaneous investigations allowed consistent comparisons between native and decellularized tissues and added analytical knowledge crucial for designing realistic constitutive models. We have provided a quantitative structural foundation for earlier biomechanical findings in pulmonary leaflets and the basis for validation of theoretical assumptions still lacking the support of experimental evidence in both conduits. Future insights into the distribution of load-bearing components in human conduits are likely to provide indications important to optimize the surgical positioning of valvular grafts.

Item Type:Article
ID Code:5578
Status:Published
Refereed:Yes
Uncontrolled Keywords:Aortic and pulmonary valve, decellularization, collagen and elastin, Glycosaminoglycans and hydration, volume fraction
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze fisiologiche, biochimiche e cellulari
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Elsevier
eISSN:1878-7568
Copyright Holders:© 2010 Acta Materialia Inc. Published by Elsevier
Deposited On:07 Mar 2011 18:37

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page