titoli, abstracts, parole chiave >>>
Application of a biomarker–transcriptomics approach utilizing mussels in the evaluation of the water quality in a marine coastal area of Medio Campidano (Sardinia, Italy)

Negri, Alessandro and Dondero, Francesco and Dagnino, Alessandro and Boatti, Lara and Sforzini, Susanna and Caprì, Flavia Giusy and Oliveri, Caterina and Viarengo, Aldo Giuseppe and Chessa, Lorenzo Antonio (2010) Application of a biomarker–transcriptomics approach utilizing mussels in the evaluation of the water quality in a marine coastal area of Medio Campidano (Sardinia, Italy). Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology, Vol. 157 (Supplement 1), p. S25-S25. eISSN 1531-4332. Article.

Full text not available from this repository.

DOI: 10.1016/j.cbpa.2010.06.072


A systems toxicology approach based on combination of biomarkers and transcriptomics analyses was used to assess the occurrence of a stress syndrome in mussels caged in proximity of a decommissioned mine site in marine coastal area of Medio Campidano (Sardinia, Italy). Mussels (M. galloprovincialis) were caged for 28 days in front of the Piscinas stream, supposed to drain pollutants from the abandoned mine sites (PIS), and in a reference site (CTR). The health status of transplanted mussels was determined involving a battery of nine biomarkers (stress, exposure and genotoxicity biomarkers) and applying an Expert System of Classification. Gene expression profiles were evaluated using a cDNA-microarray encompassing 1.7K sequences. The expression of mt10 and mt20 genes was further investigated in quantitative PCR analysis. Results showed a mild stress in the animals sampled at PIS: data show significant alterations (Mann–Whitney U-test, p < 0.05) in Lysosomal-Membrane-Stability, Neutral-Lipid and Metallothionein content, suggesting a moderate stress induced by heavy metals exposure. The transcriptomic analysis did not show the modulation of genes involved in detoxification processes, but we found two putative sequences implicated in antibacterial humoral response and other genes involved in developmental processes. RT-Q-PCR data showed a stimulated transcription of the mt20 gene in mussels from PIS. Finally, data on bioaccumulation indicated very high levels of Pb (12 fold) and other metals at a lower extent (Fe, Mn and Cd) in the animals sampled at PIS, while PAHs were below the detection levels. This complex of data seems to indicate a possible risk for both humans and the ecosystem mainly related to the leakage of metals – mainly Pb – from the decommissioned site.

Item Type:Article
ID Code:5531
Uncontrolled Keywords:M. galloprovincialis, biomarkers, stress syndrome in mussels, marine pollution, Medio Campidano (Sardinia)
Subjects:Area 05 - Scienze biologiche > BIO/07 Ecologia
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto per lo studio degli ecosistemi, Sassari
Additional Information:Work presented at the 27th Congress of the new european society of comparative biochemistry and physiology, Alessandria, Italy, September 5-9, 2010 on Biological effects of climatic changes and pollution: from biomarkers to system biology.
Deposited On:16 Mar 2011 16:50

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page