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In vivo, morphological and clinical effects of a desensitizing agent

Milia, Egle Patrizia and Cotti, Elisabetta and Sotgiu, Giovanni and Castelli, G. and Masarin, M. and Manunta, A. and Gallina, Giuseppe (2010) In vivo, morphological and clinical effects of a desensitizing agent. Dental Materials, Vol. 26 (Supplement 1), p. e67-e68. eISSN 1879-0097. Article.

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DOI: 10.1016/j.dental.2010.08.153


Objectives: This study evaluated the in vivo effectiveness of Universal Dentin Sealant (UDS), a new resin-based material, as dentinal desensitizing agent on dentin morphology and clinical symptoms.
Materials and methods: Thirty premolars, exhibiting non-carious cervical lesions, and scheduled for extraction for periodontal reasons, were selected for the ultrastructural study. These samples were randomly divided into three groups (n = 10): group 1, brushing with UDS; group 2, brushing with Flor-Opal® Varnish (FOV); and group 3, untreated control. After 7 days, teeth were extracted and samples processed for SEM and TEM comparative observations. The in vivo study was carried out on 90 teeth with non-carious cervical lesions. The assessment method used to quantify sensitivity was the cold air syringe, recorded using the visual analogue scale (VAS), prior to treatment (baseline) and after 7 days. Teeth were randomly assigned to three groups (n = 30): group 1, UDS; group 2, FOV; and group 3, untreated control. Analysis was based on the Student's t-test for paired data, with a 5% significance level.
Results: The ultrastructure of UDS/dentin interface was characterized by a superficial porous layer of spherical clusters immersed in a loose matrix that fully covered the superficial dentin. A poorly crystalline aspect of peritubular and intertubular fibrils with sealed tubular orifices was observed. The FOV/dentin interface showed an irregular layer of precipitates with partial exposure of the dentin surface. The presence of intratubular crystals partially occluding the tubular orifices and densely mineralized peritubular fibrils was also noted. Untreated control dentin showed a superficial thin layer of smear with porous plugs within the tubular orifices. Comparing clinical results, the statistical analysis revealed significant differences between both the tested materials (p < 0.001) and the control group (p > 0.05) in term of the periods of examination.
Conclusions: The application of UDS resulted in effective and stable dentinal tubular occlusion, leading to a statistically significant decrease in clinical discomfort of particular relevance to cases of dentinal hypersensitivity.

Item Type:Article
ID Code:5514
Uncontrolled Keywords:UDS and FOV/dentin, dentinal desensitizing agent, dentinal hypersensitivity
Subjects:Area 06 - Scienze mediche > MED/01 Statistica medica
Divisions:001 Università di Sassari > 03 Istituti > Igiene e medicina preventiva
Additional Information:Abstracts of the Academy of Dental Materials Annual Meeting, 7-9 October 2010, Trieste, Italy.
Deposited On:11 Mar 2011 19:23

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