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Quantitative assay for bradykinin in rat plasma by liquid chromatography coupled to tandem mass spectrometry

Baralla, Elena and Nieddu, Maria and Boatto, Gianpiero and Varoni, Maria Vittoria and Palomba, Domenico and Demontis, Maria Piera and Pasciu, Valeria and Anania, Vittorio Domenico (2011) Quantitative assay for bradykinin in rat plasma by liquid chromatography coupled to tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, Vol. 54 (3), p. 557-561. eISSN 1873-264X. Article.

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DOI: 10.1016/j.jpba.2010.09.041

Abstract

An assay to quantify bradykinin in rat plasma has been developed and validated, using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). Sar-d-Phe8-des-Arg9-bradykinin was used as internal standard. Aprotinin was added to rat plasma to inhibit the activity of proteinases. Recoveries for solid-phase extraction (SPE) on Strata X reversed phase were greater than 80%. Multiple reaction monitoring (MRM) on a triple quadrupole mass spectrometer equipped with an electrospray source (ESI), operating in the positive ion-mode, was used for detection. The assay was validated and stability was explored. Bradykinin (10–500 ng/mL) was quantified with accuracy values (% RE) below 10% and intra- and inter-day precisions (% RSD) below 12 and 16%, respectively, for all concentrations. The method was successfully applied to several plasma samples from low levels kallikrein rats (LKRs) compared with normal kallikrein rats (NKRs).

Item Type:Article
ID Code:5496
Status:Published
Refereed:Yes
Uncontrolled Keywords:Bradykinin, rat plasma, LC–MS/MS, kallikrein, SPE
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/07 Farmacologia e tossicologia veterinaria
Area 03 - Scienze chimiche > CHIM/08 Chimica farmaceutica
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
001 Università di Sassari > 01 Dipartimenti > Farmaco, chimico, tossicologico
Publisher:Elsevier
eISSN:1873-264X
Copyright Holders:© 2010 Elsevier
Deposited On:04 Mar 2011 16:40

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