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Polypeptide binding to mesostructured titania films

Mura, Stefania and Greppi, Gianfranco and Roggio, Anna Maria and Malfatti, Luca and Innocenzi, Plinio (2011) Polypeptide binding to mesostructured titania films. Microporous and Mesoporous Materials, Vol. 142 (1), p. 1-6. ISSN 1387-1811. Article.

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DOI: 10.1016/j.micromeso.2010.10.047

Abstract

Mesoporous titania films have been prepared via evaporation induced self-assembly and used as a matrix to bind linear pentapeptides. The mesoporous surface has been functionalized with amine groups by immersing the samples in a solution of aminopropyltriethoxysilane in toluene. Different pentapeptides have been synthesized for binding to the mesoporous films: H–Ile-Gln-Asp-Leu-Phe–COOH, H–Val-Gln-Asp-Leu-Phe–COOH and Fmoc-Phg-Gln-Asp-Leu-Phe–COOH. The peptides have been bonded to the amine functionalized surface of the titania mesoporous films with an impregnation process. The H–Val-Gln-Asp-Leu-Phe–COOH peptide has been successfully bonded to the titania matrix, while the other peptides have shown not be suitable for the process because of lower solubility and sterical hindrance. The functionalization with aminopropyltriethoxysilane and peptide binding has been studied by Fourier transform infrared spectroscopy and fluorescence spectroscopy. A fluorescent marker, fluorescein isothiocyanate, has been used to confirm the incorporation of the peptides into the titania matrix. The process is reliable and robust, after several washing cycle of the samples, the peptides are still well bonded to the titania mesoporous films.

Item Type:Article
ID Code:5488
Status:Published
Refereed:Yes
Uncontrolled Keywords:Self-assembly, mesoporous, films, peptide
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
002 Altri enti e centri di ricerca del Nord Sardegna > INSTM-Consorzio Interuniversitario Nazionale per la Scienza e la Tecnologia dei Materiali, Unità di ricerca di Sassari
Publisher:Elsevier
ISSN:1387-1811
Copyright Holders:© 2010 Elsevier
Deposited On:04 Mar 2011 16:09

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