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Chromosomal localisation and genetic variation of the SLC11A1 gene in goats (Capra hircus)

Vacca, Giuseppe Massimo and Pazzola, Michele and Pisano, C. and Carcangiu, Vincenzo and Diaz, M. L. and Nieddu, Mariella and Robledo, Renato and Mezzanotte, Roberto and Dettori, Maria Luisa (2011) Chromosomal localisation and genetic variation of the SLC11A1 gene in goats (Capra hircus). The Veterinary Journal, Vol. 190 (1), p. 60-65. eISSN 1532-2971. Article.

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DOI: 10.1016/j.tvjl.2010.09.028

Abstract

The solute carrier family 11 member A1 (SLC11A1) gene is associated with resistance to infectious diseases. Chromosomal localisation, genomic regions corresponding to functional domains and the genetic variability of microsatellites in the 3′ untranslated region (3′-UTR) of this gene were investigated in 427 goats (Capra hircus) of six breeds. Using dual colour fluorescence in situ hybridisation, SLC11A1 was localised to goat chromosome 2. Single strand conformation polymorphism was used to screen for polymorphisms in SLC11A1 exons 2, 10 and 15. There was no variation among goat breeds in the sarcoma homology 3 (SH3) binding motif, the protein kinase C phosphorylation site or the two N-linked glycosylation sites. Exon 15 exhibited variability due to the presence of two polymorphic microsatellites. Genotyping of the upstream guanine–thymine repeat (GTn) at 3′-UTR revealed eight alleles (GT11, GT12, GT14–GT19) in goats, whereas GT13 (present in cattle) was absent. Most goats carried the GT16 allele and no allele was found to be exclusive to only one breed. The coefficient of genetic differentiation value (GST) was 0.084. This microsatellite appears to be an informative DNA marker for genetic linkage analysis in goats.

Item Type:Article
ID Code:5484
Status:Published
Refereed:Yes
Uncontrolled Keywords:Goat, SLC11A1, chromosome, microsatellite, polymorphism
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 07 - Scienze agrarie e veterinarie > AGR/19 Zootecnica speciale
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Elsevier
eISSN:1532-2971
Deposited On:21 Feb 2011 11:00

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