Zinellu, Angelo and Sotgia, Salvatore and Scanu, Bastianina and Pisanu, Elisabetta and Sanna, Manuela and Usai, Maria Franca and Deiana, Luca and Carru, Ciriaco (2010) Ultra-fast adenosine 5′-triphosphate, adenosine 5′-diphosphate and adenosine 5′-monophosphate detection by pressure-assisted capillary electrophoresis UV detection. Electrophoresis, Vol. 31 (16), p. 2854-2857. eISSN 1522-2683. Article.
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Herein, we report a new CE method to measure adenine nucleotides adenosine 5′-triphosphate, adenosine 5′-diphosphate, and adenosine 5′-monophosphate in red blood cells. For this purpose, 20 mmol/L sodium acetate buffer at pH 3.80 was used as running electrolyte, and the separation was performed by the simultaneous application of a CE voltage of 25 kV and an overimposed pressure of 0.2 psi from inlet to outlet. A rapid separation of these analytes in less than 1.5 min was obtained with a good reproducibility for intra- and inter-assay (CV<4 and 8%, respectively) and an excellent analytical recovery (from 98.3 to 99%). The applicability of our method was proved by measuring adenine nucleotides in red blood cells.
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