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Ultra-fast adenosine 5′-triphosphate, adenosine 5′-diphosphate and adenosine 5′-monophosphate detection by pressure-assisted capillary electrophoresis UV detection

Zinellu, Angelo and Sotgia, Salvatore and Scanu, Bastianina and Pisanu, Elisabetta and Sanna, Manuela and Usai, Maria Franca and Deiana, Luca and Carru, Ciriaco (2010) Ultra-fast adenosine 5′-triphosphate, adenosine 5′-diphosphate and adenosine 5′-monophosphate detection by pressure-assisted capillary electrophoresis UV detection. Electrophoresis, Vol. 31 (16), p. 2854-2857. eISSN 1522-2683. Article.

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DOI: 10.1002/elps.201000138

Abstract

Herein, we report a new CE method to measure adenine nucleotides adenosine 5′-triphosphate, adenosine 5′-diphosphate, and adenosine 5′-monophosphate in red blood cells. For this purpose, 20 mmol/L sodium acetate buffer at pH 3.80 was used as running electrolyte, and the separation was performed by the simultaneous application of a CE voltage of 25 kV and an overimposed pressure of 0.2 psi from inlet to outlet. A rapid separation of these analytes in less than 1.5 min was obtained with a good reproducibility for intra- and inter-assay (CV<4 and 8%, respectively) and an excellent analytical recovery (from 98.3 to 99%). The applicability of our method was proved by measuring adenine nucleotides in red blood cells.

Item Type:Article
ID Code:5482
Status:Published
Refereed:Yes
Uncontrolled Keywords:Adenosine 5′-diphosphate, adenosine 5′-monophosphate, adenosine 5′-triphosphate, CE, pressure assisted
Subjects:Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Blackwell / Wiley
eISSN:1522-2683
Deposited On:11 Feb 2011 09:20

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