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A Plasmid DNA immunogen expressing fifteen protein antigens and complex virus-like particles (VLP+) mimicking naturally occurring HIV

Somogyi, Eszter and Xu, Jianquin and Gudics, Ágnes and Tóth, József and Kovács, Attila Lajos and Lori, Franco and Lisziewicz, Julianna (2011) A Plasmid DNA immunogen expressing fifteen protein antigens and complex virus-like particles (VLP+) mimicking naturally occurring HIV. Vaccine, Vol. 29 (4), p. 744-753. eISSN 1873-2518. Article.

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DOI: 10.1016/j.vaccine.2010.11.019

Abstract

We describe here a single plasmid DNA immunogen representing the broadest antigen repertoire among HIV vaccine candidates. This pDNA was “ANTIGENeered” for the regulated expression of thirteen complete and two non-functional HIV protein antigens. These proteins self assemble into complex virus-like particles (VLP+). Multiple irreversible safety features were introduced by genetic modifications including the complete impairment of integration, reverse transcription, the function of Nef and the 3′LTR. Epitope analysis predicted that the pDNA-derived protein repertoire can potentially present over 3000 T cell epitopes. However, the expressed antigens have different potential to induce HIV-specific CD4+ and CD8+ T cells supporting our hypothesis that HIV vaccines should contain all possible regulatory and structural proteins. This immunogen is the active pharmaceutical ingredient of DermaVir, a therapeutic vaccine product candidate that recently successfully completed Phase II clinical trials and meets the safety, immunogenicity and cost requirements of an HIV vaccine.

Item Type:Article
ID Code:5465
Status:Published
Refereed:Yes
Uncontrolled Keywords:DermaVir, therapeutic vaccine, HIV immunotherapy
Subjects:Area 06 - Scienze mediche > MED/17 Malattie infettive
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > ViroStatics, Sassari
Publisher:Elsevier
eISSN:1873-2518
Copyright Holders:© 2010 Elsevier
Deposited On:18 Feb 2011 13:27

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