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The Sheep milk fat globule membrane proteome

Pisanu, Salvatore and Ghisaura, Stefania and Pagnozzi, Daniela and Biosa, Grazia and Tanca, Alessandro and Roggio, Tonina and Uzzau, Sergio and Addis, Maria Filippa (2011) The Sheep milk fat globule membrane proteome. Journal of Proteomics, Vol. 74 (3), p. 350-358. eISSN 1876-7737. Article.

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DOI: 10.1016/j.jprot.2010.11.011

Abstract

Milk fat globule membranes (MFGM) are three-layered structures that enclose fat droplets, and are composed by an internal monolayer of endoplasmic reticulum origin, surrounded by a bilayer derived from the apical membrane of the lactating cell. In this work, an optimized protein extraction method was applied to sheep MFGM, and extracts were subjected to SDS-PAGE separation followed by shotgun LC tandem mass spectrometry (GeLC-MS/MS) for identification and characterization. In total, 140 unique sheep MFGM proteins (MFGMPs) were identified. All protein identification data were subjected to Gene Ontology (GO) classification for localization and function. Moreover, the relative abundance of all identified MFGMPs was estimated by means of the normalized spectral abundance factor (NSAF) approach, and GO abundance classes were obtained. The data gathered in this work provide a detailed picture of the proteome expressed in healthy sheep MFGs, and lay the foundations for future studies on sheep lactation physiology and on its alterations in pathological conditions.

Item Type:Article
ID Code:5442
Status:Published
Refereed:Yes
Uncontrolled Keywords:Sheep milk, milk fat globule membrane, GeLC-MS/MS, normalized spectral abundance, ontology
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > Porto Conte Ricerche, Alghero
002 Altri enti e centri di ricerca del Nord Sardegna > Biosistema s.c.r.l.-Centro di competenza e trasferimento tecnologico biosistema, Sassari
Publisher:Elsevier
eISSN:1876-7737
Copyright Holders:© 2010 Elsevier
Deposited On:11 Mar 2011 12:32

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