Bocca, Beatrice and Madeddu, Roberto Beniamino and Asara, Yolande and Tolu, Paola and Marchal Corrales, Juan Antonio and Forte, Giovanni (2011) Assessment of reference ranges for blood Cu, Mn, Se and Zn in a selected Italian population. Journal of Trace Elements in Medicine and Biology, Vol. 25 (1), p. 19-26. eISSN 1878-3252. Article.
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The reference ranges for blood Cu, Mn, Se and Zn of 215 adult subjects non-occupationally exposed and living in the Nuoro province (Sardinia, insular Italy) were assessed. Metals were determined by sector field inductively coupled plasma mass spectrometry after microwave-assisted acid digestion of blood. The blood reference ranges estimated as P5–P95 percentiles (geometric mean, GM) were 776–1495 μg/L (1036 μg/L) for Cu; 4.73–17.0 μg/L (8.91 μg/L) for Mn; 106–185 μg/L (140 μg/L) for Se and 4686–8585 μg/L (6418 μg/L) for Zn. These results were then stratified for sex, age, alcohol consumption, smoking habit and living area. The GM value of Cu was significantly higher in females (1127 μg/L) than in males (957 μg/L). Age, alcohol intake, smoking habit and residential area did not influence blood Cu. The GM of Mn was significantly higher in females (9.98 μg/L) respect to males (8.01 μg/L) and in drinkers (9.67 μg/L) compared to non-drinkers (8.38 μg/L). The other variables did not change the Mn data. Selenium concentrations did not differ significantly as a function of individuals factors. With reference to Zn, males had GM of 6804 μg/L and females of 6031 μg/L, and more blood Zn was found in subjects consuming alcohol (6618 μg/L) respect to abstainers (6155 μg/L). In addition, blood Zn was not affected by age, smoking habit and place of living.
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