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Comparative performance of three TSE rapid tests for surveillance in healthy sheep affected by scrapie

Bozzetta, Elena and Nappi, Raffaella and Crudeli, Silvia and Meloni, Daniela and Varello, Katia and Loprevite, Daniela and Melis, Paola Grazia and Mazza, Maria and Colussi, Silvia and Ingravalle, Francesco and Ru, Giuseppe and Nonno, Romolo and Ligios, Ciriaco (2011) Comparative performance of three TSE rapid tests for surveillance in healthy sheep affected by scrapie. Journal of Virological Methods, Vol. 173 (2), p. 161-168. eISSN 1879-0984. Article.

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DOI: 10.1016/j.jviromet.2011.01.008


Rapid tests specific for sheep and goats became part of European Union-wide active scrapie surveillance in 2006. Performance of three approved TSE rapid tests for the detection of sheep infected with scrapie in field cases in the pre-clinical stage of the disease was compared.
The medulla oblongata of 969 asymptomatic sheep of various genotype and breed aged over 18 months from 23 Italian flocks affected with scrapie, were tested by the Bio-Rad TeSeE Sheep/Goat (A), the IDEXX HerdChek BSE-Scrapie Antigen Test Kit, EIA (B) and the Prionics®-Check Western Small Ruminant (C) rapid tests.
Of 136 positive samples of classical scrapie, as confirmed by Western blot assay, 132 were positive with test A (Se 97.06%, CI 95% 92.64–99.19); 135 with test B (Se 99.26%, 95% CI 95.97–99.98) and 128 with test C (Se 94.12%, 95% CI 88.74–97.43).
Tests A and B showed the best performance on analytical sensitivity. All three systems demonstrated good reproducibility: being the intrarater and interrater kappa coefficients always over 0.83.
The one available atypical scrapie sample was positive with tests A and B, negative with test C.
Considering the discrepant results in the detection of low PrPsc concentrations and of the atypical case, differences can be expected in the efficacy of an active surveillance system, depending on the test adopted.

Item Type:Article
ID Code:5422
Uncontrolled Keywords:TSE rapid tests, sheep, diagnostic sensitivity, reproducibility
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/03 Patologia generale e anatomia patologica veterinaria
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zooprofilattico sperimentale della Sardegna, Sassari
Deposited On:14 Feb 2011 08:55

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