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RP-HPLC determination of water-soluble vitamins in honey

Ciulu, Marco and Solinas, Silvia and Floris, Ignazio and Panzanelli, Angelo and Pilo, Maria Itria and Piu, Paola Costantina and Spano, Nadia and Sanna, Gavino (2011) RP-HPLC determination of water-soluble vitamins in honey. Talanta, Vol. 83 (3), p. 924-929. eISSN 1873-3573. Article.

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DOI: 10.1016/j.talanta.2010.10.059


The assessment and validation of reliable analytical methods for the determination of vitamins in sugarbased matrices (e.g. honey) are still scarcely explored fields of research. This study proposes and fully validates a simple and fast RP-HPLC method for the simultaneous determination of five water-soluble vitamins (vitamin B2, riboflavin; vitamin B3, nicotinic acid; vitamin B5, pantothenic acid; vitamin B9, folic acid; and vitamin C, ascorbic acid) in honey. The method provides low detection and quantification limits, very good linearity in a large concentration interval, very good precision, and the absence of any bias. It has been successfully applied to 28 honey samples (mainly from Sardinia, Italy) of 12 different botanical origins. While the overall amount of the analytes in the samples is quite low (always below 40mgkg−1), we have observed a marked dependence of some of their concentrations (i.e. vitamin B3 and vitamin B5) and the botanical origin of the honey. This insight might lead to important characterization features for this food item.

Item Type:Article
ID Code:5407
Uncontrolled Keywords:Honey, water-soluble vitamins, B-group vitamins, C vitamin, RP-HPLC
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/11 Entomologia generale e applicata
Area 03 - Scienze chimiche > CHIM/01 Chimica analitica
Divisions:001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
001 Università di Sassari > 01 Dipartimenti > Chimica
Copyright Holders:© 2010 Elsevier
Deposited On:31 Jan 2011 12:47

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