titoli, abstracts, parole chiave >>>
Potential role for IL-2 ELISpot in differentiating recent and remote infection in tuberculosis contact tracing

Krummel, Benjamin and Strassburg, Alan and Ernst, Martin and Reiling, Norbert and Eker, Barbara and Rath, Heidrun and Hörster, Robert and Wappler, Waltraud and Glaewe, Andrea and Schöllhorn, Volker and Sotgiu, Giovanni and Lange, Christoph (2010) Potential role for IL-2 ELISpot in differentiating recent and remote infection in tuberculosis contact tracing. PLoS One, Vol. 5 (7), e11670. ISSN 1932-6203. Article.

Full text disponibile come PDF Richiede visualizzatore di PDF come GSview, Xpdf o Adobe Acrobat Reader
Available under License Creative Commons Attribution.


DOI: 10.1371/journal.pone.0011670


Interferon (IFN)-γ release assays (IGRA) have improved tuberculosis contact tracing, but discrimination of recent from remote Mycobacterium tuberculosis contacts is not possible by IGRA alone. We present results of a tuberculosis contact investigation with a new early-secretory-antigenic-target (ESAT)-6 and culture-filtrate-protein (CFP)-10 specific interleukin (IL)-2 ELISpot in addition to ESAT-6 and CFP-10 specific IFN-γ ELISpot and tuberculin skin testing (TST). Results of the TST, IFN-γ ELISpot and IL-2 ELISpot were positive in 6/172 (3.4%), 7/167 (4.2%) and 6/196 (3.1%) of contacts, respectively. Close contact (≥100 hours) to the index case increased the risk of positive results in the IFN-γ ELISpot, TST, and IL-2 ELISpot by 40.8, 19.3, and 2.5 times, respectively. Individuals with a positive IFN-γ ELISpot/negative IL-2 ELISpot result had a median (IQR) duration of index case exposure of 568 hours (133_1000) compared to individuals with a positive IFN-γ ELISpot/positive IL-2 ELISpot result (median=24 hours; 20_130; p-value=0.047). Combination of a M. tuberculosis specific IFN-γ ELISpot with a M. tuberculosis specific IL-2 ELISpot significantly improved the identification of individuals with the highest risk of recent M. tuberculosis infection and is a promising method that should be explored to target tuberculosis preventive chemotherapy.

Item Type:Article
ID Code:5380
Uncontrolled Keywords:Mycobacterium tuberculosis, Enzyme-linked immunosorbent spot (ELISPOT) results, tuberculin skin test
Subjects:Area 06 - Scienze mediche > MED/01 Statistica medica
Divisions:001 Università di Sassari > 03 Istituti > Igiene e medicina preventiva
Publisher:Public Library of Science
Copyright Holders:© 2010 Krummel et al.
Deposited On:28 Jan 2011 09:58

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page