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Molecular alterations in key-regulator genes among patients with T4 breast carcinoma

Massidda, Bruno and Sini, Maria Cristina and Budroni, Mario and Atzori, Francesco and Deidda, Maria Cristina and Pusceddu, Valeria and Perra, Maria Teresa and Sirigu, Paola and Cossu, Antonio and Palomba, Grazia and Ionta, Maria Teresa and Palmieri, Giuseppe (2010) Molecular alterations in key-regulator genes among patients with T4 breast carcinoma. BMC Cancer, Vol. 10 , p. 1-10. ISSN 1471-2407. Article.

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DOI: 10.1186/1471-2407-10-458

Abstract

Background: Prognostic factors in patients who are diagnosed with T4 breast carcinomas are widely awaited. We here evaluated the clinical role of some molecular alterations involved in tumorigenesis in a well-characterized cohort of T4 breast cancer patients with a long follow-up period.

Methods: A consecutive series of 53 patients with T4 breast carcinoma was enrolled between 1992 and 2001 in Sardinia, and observed up for a median of 125 months. Archival paraffin-embedded tissue sections were used for immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) analyses, in order to assess alterations in expression levels of survivin, p53, and pERK1-2 proteins as well as in amplification of CyclinD1 and h-prune genes. The Kaplan-Meier and Cox regression methods were used for survival assessment and statistical analysis.

Results: Overall, patients carrying increased expression of pERK1-2 (p = 0.027) and survivin (p = 0.008) proteins as well as amplification of h-prune gene (p = 0.045) presented a statistically-significant poorer overall survival in comparison with cases found negative for such alterations. After multivariate analysis, the pathological response to primary chemotherapy and the survivin overexpression in primary carcinoma represented the main parameters with a role as independent prognostic factors in our series.

Conclusions: Although retrospective, our study identified some molecular parameters with a significant impact on prediction of the response to therapy or prognosis among T4 breast cancer patients. Further large prospective studies are needed in order to validate the use of such markers for the management of these patients.

Item Type:Article
ID Code:5371
Status:Published
Refereed:Yes
Uncontrolled Keywords:T4 breast carcinomas, immunohistochemistry (IHC), Fluorescence In Situ Hybridization (FISH) analyses, Sardinia
Subjects:Area 06 - Scienze mediche > MED/06 Oncologia medica
Area 05 - Scienze biologiche > BIO/16 Anatomia umana
Divisions:001 Università di Sassari > 03 Istituti > Anatomia patologica
002 Altri enti e centri di ricerca del Nord Sardegna > Azienda ASL1, Sassari > Servizio di anatomia patologica
002 Altri enti e centri di ricerca del Nord Sardegna > CNR-Consiglio Nazionale delle Ricerche > Istituto di chimica biomolecolare, Sassari
002 Altri enti e centri di ricerca del Nord Sardegna > Azienda ASL1, Sassari > Centro multizonale di osservazione epidemiologica > Registro tumori di Sassari
Publisher:BioMed Central
ISSN:1471-2407
Copyright Holders:© 2010 Massidda et al.; licensee BioMed Central Ltd.
Deposited On:28 Jan 2011 08:15

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