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Ni(II) and Cu(II) binding with a 14-aminoacid sequence of Cap43 protein, TRSRSHTSEGTRSR

Zoroddu, Maria Antonietta and Kowalik-Jankowska, Teresa and Kozlowski, Henryk and Salnikow, Konstantin and Costa, Max (2001) Ni(II) and Cu(II) binding with a 14-aminoacid sequence of Cap43 protein, TRSRSHTSEGTRSR. Journal of Inorganic Biochemistry, Vol. 84 (1-2), p. 47-54. ISSN 0162-0134. Article.

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DOI: 10.1016/S0162-0134(00)00204-X

Abstract

The tetradecapeptide containing the 10 aminoacid repeated sequence on the C-terminus of the Ni(II)-induced Cap43 protein, was analyzed for Ni(II) and Cu(II) binding. A combined pH-metric and spectroscopic UV–VIS, EPR, CD and NMR study of Ni(II) and Cu(II) binding to the blocked CH3CO-Thr-Arg-Ser-Arg-Ser-His-Thr-Ser-Glu-Gly-Thr-Arg-Ser-Arg-NH2 (Ac-TRSRSHTSEGTRSR-Am) peptide, modeling a part of the C-terminal sequence of the Cap43 protein, revealed the formation of octahedral complexes involving imidazole nitrogen of histidine, at pH 5.5 and pH 7 for Cu(II) and Ni(II), respectively; a major square planar 4N–Ni(II) complex (about 100% at pH 9, log K*=−28.16) involving imidazole nitrogen of histidine and three deprotonated amide nitrogens of the backbone of the peptide was revealed; a 3N–Cu(II) complex (maximum about 70% at pH 7, log K*=−13.91) and a series of 4N–Cu(II) complexes starting at pH 5.5 (maximum about 90% at pH 8.7, log K*=−21.39 for CuH−3L), were revealed. This work supports the existence of a metal binding site at the COOH-terminal part of the Cap43 peptide.

Item Type:Article
ID Code:5306
Status:Published
Refereed:Yes
Uncontrolled Keywords:Ni(II), Cu(II), binding, Cap43, 14-aminoacid peptide
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
Publisher:Elsevier Science
ISSN:0162-0134
Additional Information:Erratum: "The headline on the title page and the running headlines on all other pages (first lines on top) have been represented wrongly. Instead of volume 84, volume 85 has been typed".
Deposited On:12 Jan 2011 10:05

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