Zoroddu, Maria Antonietta and Kowalik-Jankowska, Teresa and Kozlowski, Henryk and Salnikow, Konstantin and Costa, Max (2001) Ni(II) and Cu(II) binding with a 14-aminoacid sequence of Cap43 protein, TRSRSHTSEGTRSR. Journal of Inorganic Biochemistry, Vol. 84 (1-2), p. 47-54. ISSN 0162-0134. Article.
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The tetradecapeptide containing the 10 aminoacid repeated sequence on the C-terminus of the Ni(II)-induced Cap43 protein, was analyzed for Ni(II) and Cu(II) binding. A combined pH-metric and spectroscopic UV–VIS, EPR, CD and NMR study of Ni(II) and Cu(II) binding to the blocked CH3CO-Thr-Arg-Ser-Arg-Ser-His-Thr-Ser-Glu-Gly-Thr-Arg-Ser-Arg-NH2 (Ac-TRSRSHTSEGTRSR-Am) peptide, modeling a part of the C-terminal sequence of the Cap43 protein, revealed the formation of octahedral complexes involving imidazole nitrogen of histidine, at pH 5.5 and pH 7 for Cu(II) and Ni(II), respectively; a major square planar 4N–Ni(II) complex (about 100% at pH 9, log K*=−28.16) involving imidazole nitrogen of histidine and three deprotonated amide nitrogens of the backbone of the peptide was revealed; a 3N–Cu(II) complex (maximum about 70% at pH 7, log K*=−13.91) and a series of 4N–Cu(II) complexes starting at pH 5.5 (maximum about 90% at pH 8.7, log K*=−21.39 for CuH−3L), were revealed. This work supports the existence of a metal binding site at the COOH-terminal part of the Cap43 peptide.
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