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Effect of buserelin administered 24 hours prior to A.I. in Sarda ewes: LH, 17β-oestradiol and progesterone plasma levels

Marongiu, Maria Laura and Branca, Andrea and Gallus, Marilia and Pelliccia, C. and Bomboi, Giovanni Cristoforo and Floris, Basilio Remo (2002) Effect of buserelin administered 24 hours prior to A.I. in Sarda ewes: LH, 17β-oestradiol and progesterone plasma levels. Reproduction in Domestic Animals, Vol. 37 (4), p. 242-243. eISSN 1439-0531. Article.

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DOI: 10.1046/j.1439-0531.2002.00380.x


Buserelin administered 24 h prior to A.I. increased ovulation rates in Sarda ewe. This study reports the effect of the same treatment on LH, E2 and P4 plasma levels. After a classic synchronization treatment of oestrous cycles, 24 multiparous Sarda ewes were submitted to A.I. (Day 0) 55 h after sponges removal. On Day – 1 12 ewes (Group T) were given a single i.m. injection of 4 μg/progestin buserelin (Receptal) and the remaining 12 ewes (Group C) acted as control. Blood samples were collected from each subject at 2-h intervals on Days – 1 and 0 (from 8:00 to 16:00 h) and twice on Day + 1 (8:00 and 15:00 h). LH concentration was assessed by ELISA (Reprokit), E2 and P4 by homologous radioimmunoassay. Buserelin caused in T subjects, compared to controls, an increase of LH (Day – 1), a decrease of E2 (Days – 1 and 0) and an increase of P4 concentrations (Day + 1). These differences were statistically significant (P < 0:05). The results are in agreement to our previous observations on follicular dynamics: the higher LH and P4 levels reflected the increased number of corpora lutea and the lower E2 level the decreased number of large follicles.

Item Type:Article
ID Code:5258
Uncontrolled Keywords:Poster presented at the The 6th Annual Meeting of the European Society for Domestic Animal Reproduction (ESDAR), Parma, Italy, 12–14 September 2002.
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/20 Zoocolture
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze zootecniche
002 Altri enti e centri di ricerca del Nord Sardegna > Istituto zootecnico e caseario per la Sardegna, Olmedo
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Blackwell / Wiley
Copyright Holders:© 2002 Blackwell
Deposited On:17 Dec 2010 16:30

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