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Molecular characterisation of vegetative compatibility groups in Fusarium oxysporum f. sp. radicis-lycopersici and f. sp. lycopersic by random amplification of polymorphic DNA and microsatellite-primed PCR

Balmas, Virgilio and Scherm, Barbara and Di Primo, Pietro and Rau, Domenico and Marcello, Angela and Migheli, Quirico (2005) Molecular characterisation of vegetative compatibility groups in Fusarium oxysporum f. sp. radicis-lycopersici and f. sp. lycopersic by random amplification of polymorphic DNA and microsatellite-primed PCR. European Journal of Plant Pathology, Vol. 111 (1), p. 1-8. eISSN 1573-8469. Article.

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DOI: 10.1007/s10658-004-1602-9

Abstract

Random amplification of polymorphic DNA (RAPD-PCR) analysis was conducted on 48 isolates of Fusarium oxysporum f. sp. radicis-lycopersici (F.o.r.l.) from different geographic regions, representing all known vegetative compatibility groups (VCGs) except VCG 0097 and VCG 0099 and on eight isolates of F.oxysporum f. sp. lycopersici (F.o.l.), representing VCGs 0030, 0031, 0032 and 0033. Upon UPGMA (unweighted pair-group method with arithmetic averages) analysis of 86 RAPD-PCR markers generated by 16 informative primers and 44 markers obtained with eight microsatellite primers, a close relatedness was evident for F.o.r.l. isolates in VCGs 0090, 0092, 0096, and, to a lesser extent, for those in VCG 0093. Representatives of VCG 0091 formed a distinct group, while F.o.r.l. isolates in VCGs 0094 and 0098 were not distinguishable by the tested markers, most of which were also shared by F.o.l. isolates belonging to VCGs 0031 and 0033. F.o.l. isolates in VCGs 0030 and 0032 shared most of the molecular markers. The correlation between RAPD-PCR and microsatellite genetic distance was highly significant (R2 = 0.77; P by Mantel test < 0.001). The molecular variability observed in both formae speciales is discussed in relation to the development of F.o.r.l.- and F.o.l.-specific diagnostic tools.

Item Type:Article
ID Code:513
Status:Published
Refereed:Yes
Uncontrolled Keywords:Fusarium crown and root rot, Fusarium wilt, genetic structure, phytodiagnosis, tomato
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/07 Genetica agraria
Area 07 - Scienze agrarie e veterinarie > AGR/12 Patologia vegetale
Divisions:001 Università di Sassari > 02 Centri > Centro di eccellenza interdisc. sviluppo ricerca biotecnologica e studio biodiversità della Sardegna e dell'area mediterranea
001 Università di Sassari > 01 Dipartimenti > Scienze agronomiche e genetica vegetale agraria
001 Università di Sassari > 01 Dipartimenti > Protezione delle piante
Publisher:Springer Netherlands
eISSN:1573-8469
Deposited On:18 Aug 2009 10:02

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