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Development of a voltammetric technique for monitoring brain dopamine metabolism: compensation for interference caused by DOPAC electrogenerated during homovanillic acid detection

Al-Mulla, Ismael and Lowry, John P. and Serra, Pier Andrea and O'Neill, Robert D. (2009) Development of a voltammetric technique for monitoring brain dopamine metabolism: compensation for interference caused by DOPAC electrogenerated during homovanillic acid detection. Analyst, Vol. 134 (5), p. 893-898. eISSN 1364-5528. Article.

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DOI: 10.1039/B810227A

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Abstract

The established stability of carbon-paste electrodes (CPEs) in brain extracellular fluid was exploited to develop a voltammetric technique to monitor the dopamine metabolite, homovanillic acid (HVA), at 10 s intervals. At the scan rates needed for this time resolution, 3,4-dihydroxyphenylacetic acid (DOPAC), electrogenerated as a result of HVA oxidation, was observed in the cyclic staircase voltammograms, and this interfered with the straightforward reliable quantification of HVA. However, correction of the HVA signal, recorded in mixtures, with currents from the DOPAC and ascorbate regions of the voltammogram allowed the reproducible construction of well behaved HVA calibration plots. These showed good linearity, LOD values, selectivity and stability during six days of continuous CPE exposure to a lipid medium, which served as an in-vitro model of CPE implantation in brain tissue for future applications.

Item Type:Article
ID Code:4989
Status:Published
Refereed:Yes
Uncontrolled Keywords:Dopamine detection
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:Royal Society of Chemistry
eISSN:1364-5528
Deposited On:26 Nov 2010 15:43

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