UnissResearch

Logo Universitàegli studi di Sassari
titoli, abstracts, parole chiave >>>
Real-time monitoring of brain tissue oxygen using a miniaturized biotelemetric device implanted in freely moving rats

Bazzu, Gianfranco and Puggioni, Giulia Maria Grazia and Dedola, Sonia and Calia, Giammario and Rocchitta, Gaia Giovanna Maria and Migheli, Rossana and Desole, Maria Speranza and Lowry, John P. and O’Neill, Robert D. and Serra, Pier Andrea (2009) Real-time monitoring of brain tissue oxygen using a miniaturized biotelemetric device implanted in freely moving rats. Analytical Chemistry, Vol. 81 (6), p. 2235-2241. ISSN 0003-2700. Article.

Full text not available from this repository.

DOI: 10.1021/ac802390f

Abstract

A miniaturized biotelemetric device for the amperometric detection of brain tissue oxygen is presented. The new system, derived from a previous design, has been coupled with a carbon microsensor for the real-time detection of dissolved O2 in the striatum of freely moving rats. The implantable device consists of a single-supply sensor driver, a current-to-voltage converter, a microcontroller, and a miniaturized data transmitter. The oxygen current is converted to a digital value by means of an analog-to-digital converter integrated in a peripheral interface controller (PIC). The digital data is sent to a personal computer using a six-byte packet protocol by means of a miniaturized 434 MHz amplitude modulation (AM) transmitter. The receiver unit is connected to a personal computer (PC) via a universal serial bus. Custom developed software allows the PC to store and plot received data. The electronics were calibrated and tested in vitro under different experimental conditions and exhibited high stability, low power consumption, and good linear response in the nanoampere current range. The in vivo results confirmed previously published observations on oxygen dynamics in the striatum of freely moving rats. The system serves as a rapid and reliable model for studying the effects of different drugs on brain oxygen and brain blood flow and it is suited to work with direct-reduction sensors or O2-consuming biosensors.

Item Type:Article
ID Code:4988
Status:Published
Refereed:Yes
Uncontrolled Keywords:Miniaturized biotelemetric device, rats, brain tissue, drugs
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:American Chemical Society
ISSN:0003-2700
Deposited On:26 Nov 2010 16:34

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page