Ali Ammar, Bin Talib (2012) Studies of the effect of semi-defined diluents on the cryo-tollerance of spermatozoa and in vitro production embryos. Doctoral Thesis.
In this study we focus on three main points. First,over the past 70 years, the cryoprotective media used to dilute semen for sperm storage have been continuously revised, but the basic ingredients of the media remain unchanged. We examined the effect of using semi-defined diluents on the post-thaw semen quality and in vitro production (IVP) embryos by using different concentrations of bovine serum albumin (BSA) compared with 20% egg yolk (EY). Eleven ejaculates in three months were divided equally into three diluents for the treatment and control groups. Post-thawed semen was analyzed by using six semen tests and through the IVP embryos process.Second, in vitro fertilization (IVF) techniques have continuously progressed since the 1980s. The standard IVF protocol recommended overnight gamete co-incubation. We tested the beneficial effect of reduced co-incubation times on embryo production and quality. Third, hen EY is one of the most commonly used as cryoprotectant freezing-thawing mammalian spermatozoa. We compared the effect of using different types of EY on post-thaw semen quality and IVP embryos. Semen pooled was divided equally into five different types of EY compared with hen EY diluents. Frozen-thawed spermatozoa were analyzed by using six different tests and through the IVP embryos process.
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