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Biotelemetric monitoring of brain neurochemistry in conscious rats using microsensors and biosensors

Calia, Giammario and Rocchitta, Gaia Giovanna Maria and Migheli, Rossana and Puggioni, Giulia Maria Grazia and Spissu, Ylenia and Bazzu, Gianfranco and Mazzarello, Vittorio Lorenzo Giuseppe and Lowry, John P. and O’Neill, Robert D. and Desole, Maria Speranza and Serra, Pier Andrea (2009) Biotelemetric monitoring of brain neurochemistry in conscious rats using microsensors and biosensors. Sensors, Vol. 9 (4), p. 2511-2523. ISSN 1424-8220. Article.

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DOI: 10.3390/s90402511

Abstract

In this study we present the real-time monitoring of three key brain neurochemical species in conscious rats using implantable amperometric electrodes interfaced to a biotelemetric device. The new system, derived from a previous design, was coupled with carbon-based microsensors and a platinum-based biosensor for the detection of ascorbic acid (AA), O2 and glucose in the striatum of untethered, freely-moving rats. The miniaturized device consisted of a single-supply sensor driver, a current-to-voltage converter, a microcontroller and a miniaturized data transmitter. The redox currents were digitized to digital values by means of an analog-to-digital converter integrated in a peripheral interface controller (PIC), and sent to a personal computer by means of a miniaturized AM transmitter. The electronics were calibrated and tested in vitro under different experimental conditions and exhibited high stability, low power consumption and good linear response in the nanoampere current range. The in-vivo results confirmed ON ACCESSpreviously published observations on striatal AA, oxygen and glucose dynamics recorded in tethered rats. This approach, based on simple and inexpensive components, could be used as a rapid and reliable model for studying the effects of different drugs on brain neurochemical systems.

Item Type:Article
ID Code:4984
Status:Published
Refereed:Yes
Uncontrolled Keywords:Biotelemetry, microsensor, biosensor, glucose, oxygen, ascorbic acid
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 05 - Scienze biologiche > BIO/16 Anatomia umana
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:MDPI Publishing
ISSN:1424-8220
Publisher Policy:Depositato in conformità con la politica di copyright dell'Editore
Deposited On:26 Nov 2010 15:36

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