Pirino, Giorgia and Zara, Severino and Pinna, Giovanni and Farris, Giovanni Antonio and Budroni, Marilena (2004) Diversity of Y region at HML locus in a Saccharomyces cerevisiae strain isolated from a Sardinian wine. Antonie van Leeuwenhoek, Vol. 85 (1), p. 29-36. eISSN 1572-9699. Article.
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Several mutations in genes involved in Saccharomyces mating type switching may affect the homothallic behaviour in wine yeasts. In this study the semi-homothallic (Hq) segregation of a flor wine yeast strain was analysed.We aimed to understand the molecular basis of this behaviour in a flor autochthonous strain, verifying the MAT locus status by a PCR-based HO gene disruption and sequencing of the Y region of the HML, HMR and MAT loci, after nested PCR. Presence of ORFs a1 and a2 in theYregion of the HML locus was found. At the ORF a2 at HML locus, a mutation in the stop codon was found, so the a2 ORF contains 33 more bases.
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