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PCR-based methods to discriminate Bacillus thuringiensis strains

Zara, Giacomo and Zara, Severino and Mangia, Nicoletta Pasqualina and Garau, Giovanni and Pinna, Claudia <1973- > and Ladu, Gianfranca and Budroni, Marilena (2006) PCR-based methods to discriminate Bacillus thuringiensis strains. Annals of Microbiology, Vol. 56 (1), p. 71-76. ISSN 1590-4261. Article.

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DOI: 10.1007/BF03174973

Abstract

Bacillus thuringiensis is a Gram positive bacterium that is used for the production of biopesticides. The toxic action of different strains and serovars of B. thuringiensis can be extremely selective towards specific pests, or, in contrast, it can affect a wide variety of non-target organisms such as insects, vertebrates or humans. A reliable characterization of the cultivated strains is of primary importance for the biopesticide industry, in order to assess the contamination of the final product with strains with different pesticide actions or that might be dangerous for human health. The aim of this study was to develop useful methods for the typing of different B. thuringiensis strains using two PCR-based methods, RAPD and Rep-PCR with BOXA1R and ERIC2 primers. The molecular fingerprints obtained using ERIC2-PCR showed a reliable ability to discriminate Bacillus thuringiensis strains.

Item Type:Article
ID Code:4962
Status:Published
Refereed:Yes
Uncontrolled Keywords:Bacillus thuringiensis, RAPD PCR, Rep-PCR
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze ambientali agrarie e biotecnologie agro-alimentari
Publisher:University of Milan, Department of Food Science and Microbiology
ISSN:1590-4261
Deposited On:25 Nov 2010 11:13

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