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Oxygen tolerance of an implantable polymer/enzyme composite glutamate biosensor displaying polycation-enhanced substrate sensitivity

McMahon, Colm P. and Rocchitta, Gaia Giovanna Maria and Kirwan, Sarah M. and Killoran, Sarah J. and Serra, Pier Andrea and Lowry, John P. and O'Neill, Robert D. (2007) Oxygen tolerance of an implantable polymer/enzyme composite glutamate biosensor displaying polycation-enhanced substrate sensitivity. Biosensors and Bioelectronics, Vol. 22 (7), p. 1466-1473. ISSN 0956-5663. Article.

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DOI: 10.1016/j.bios.2006.06.027

Abstract

Biosensors were fabricated at neutral pH by sequentially depositing the polycation polyethyleneimine (PEI), the stereoselective enzyme L-glutamate oxidase (GluOx) and the permselective barrier poly-ortho-phenylenediamine (PPD) onto 125-μm diameter Pt wire electrodes (Pt/PEI/GluOx/PPD). These devices were calibrated amperometrically at 0.7 V versus SCE to determine the Michaelis–Menten parameters for enzyme substrate, L-glutamate (Glu) and co-substrate, dioxygen. The presence of PEI produced a 10-fold enhancement in the detection limit for Glu (~20 nM) compared with the corresponding PEI-free configurations (Pt/GluOx/PPD), without undermining their fast response time (~2 s). Most remarkable was the finding that, although some designs of PEI-containing biosensors showed a 10-fold increase in linear region sensitivity to Glu, their oxygen dependence remained low.

Item Type:Article
ID Code:4935
Status:Published
Refereed:Yes
Uncontrolled Keywords:Glutamate oxidase, polyethyleneimine, PEI, poly(o-phenylenediamine), PPD, Michaelis–Menten, brain monitoring, neurochemistry
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Neuroscienze, scienze materno infantili
Publisher:Elsevier
ISSN:0956-5663
Deposited On:23 Nov 2010 09:35

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