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Gold(III) complexes with bipyridyl ligands: solution chemistry, cytotoxicity, and DNA binding properties

Marcon, Giordana and Carotti, Stefania and Coronnello, Marcella and Messori, Luigi and Mini, Enrico and Orioli, Pierluigi and Mazzei, Teresita and Cinellu, Maria Agostina and Minghetti, Giovanni (2002) Gold(III) complexes with bipyridyl ligands: solution chemistry, cytotoxicity, and DNA binding properties. Journal of Medicinal Chemistry, Vol. 45 (8), p. 1672-1677. eISSN 1520-4804. Article.

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DOI: 10.1021/jm010997w

Abstract

Gold(III) compounds generally exhibit significant cytotoxic effects on cancer cell lines and are of potential interest as antitumor drugs. We report here on the solution chemistry, the cytotoxicity, and the DNA binding properties of two new bipyridyl gold(III) compounds: [Au(bipy)(OH)2][PF6] (1) and the organometallic compound [Au(bipyc-H)(OH)][PF6] (2) (bipyc = 6-(1,1-dimethylbenzyl)-2,2‘-bipyridine). Both compounds are sufficiently soluble, and stable for hours, within a physiological buffer at 37 °C; [Au(bipy)(OH)2][PF6], at variance with [Au(bipyc-H)(OH)][PF6], is quickly and quantitatively reduced by ascorbate. Both compounds showed relevant cytotoxic effects toward the A2780S, A2780R, and SKOV3 tumor cell lines; lower effects were detected on the CCRF-CEM/S and CCRF-CEM/R lines. In most cases the mechanisms of resistance to CDDP are only marginally effective against these gold(III) complexes. The interactions of [Au(bipy)(OH)2][PF6] and [Au(bipyc-H)(OH)][PF6] with calf thymus DNA were investigated in vitro by various techniques to establish whether DNA represents a primary target for these compounds. Addition of saturating amounts of DNA did not affect appreciably the visible spectra of these gold(III) complexes. Some slight modifications of the CD spectra of calf thymus DNA and of the DNA melting parameters were observed; in any case, ultrafiltration experiments showed that binding of these gold(III) complexes to DNA is weak and reversible. The mechanistic implications of these findings are discussed.

Item Type:Article
ID Code:4927
Status:Published
Refereed:Yes
Uncontrolled Keywords:Antitumor agents, gold(III) complexes, cytotoxicity, bipyridyl ligands, DNA binding
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
Publisher:American Chemical Society
eISSN:1520-4804
Deposited On:22 Nov 2010 18:38

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