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Levels of human tissue kallikrein in the vitreous fluid of patients with severe proliferative diabetic retinopathy

Pinna, Antonio <1963- > and Emanueli, Costanza and Dore, Stefano and Salvo, Marco Michele and Madeddu, Paolo Roberto and Carta, Francesco (2004) Levels of human tissue kallikrein in the vitreous fluid of patients with severe proliferative diabetic retinopathy. Ophthalmologica, Vol. 218 (4), p. 260-263. ISSN 0030-3755. Article.

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DOI: 10.1159/000078617

Abstract

The aim of this study was to determine the levels of human tissue kallikrein in the vitreous fluid of patients with severe proliferative diabetic retinopathy (PDR). Tissue kallikrein levels were measured using a specific ELISA (range: 0.4–25 ng/ml) in 7 vitreous fluids from eyes with severe PDR. Seven vitreous samples from eyes which underwent vitrectomy because of rhegmatogenous retinal detachment served as non-PDR controls. Enzymatic activity was also tested by an amidolytic assay using a chromogenic substrate. In the PDR patients, vitreous tissue kallikrein was <0.4 ng/ml (5 eyes) or very low (0.52 and 0.58 ng/ml). Vitreous tissue kallikrein was <0.4 ng/ml in all non-PDR controls. These results were confirmed by the amidolytic test. Results suggest that vitreous tissue kallikrein probably plays either a secondary or no role in the pathogenesis of PDR.

Item Type:Article
ID Code:4832
Status:Published
Refereed:Yes
Uncontrolled Keywords:Vitreous, human tissue kallikrein, proliferative diabetic retinopathy, vitreous hemorrhage, pars plana vitrectomy, ELISA, amidolytic test
Subjects:Area 06 - Scienze mediche > MED/30 Malattie apparato visivo
Area 06 - Scienze mediche > MED/09 Medicina interna
Divisions:001 Università di Sassari > 03 Istituti > Clinica medica generale e terapia medica
001 Università di Sassari > 03 Istituti > Oftalmologia, Otorinolaringoiatria e Urologia
Publisher:S. Karger AG
ISSN:0030-3755
Deposited On:15 Nov 2010 14:38

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