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Multidimensional NMR spectroscopy for the study of histone H4–Ni(II) interaction

Zoroddu, Maria Antonietta and Peana, Massimiliano Francesco and Medici, Serenella (2007) Multidimensional NMR spectroscopy for the study of histone H4–Ni(II) interaction. Dalton Transactions, Vol. 2007 (3), p. 379-384. eISSN 1477-9234. Article.

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DOI: 10.1039/B610725G

Abstract

The N-terminal 30-amino acid tail of histone H4, a nuclear protein, was studied as a model for the interaction of this protein with Ni(II) ions. The behaviour of the ends-blocked Ac-SGRGKGGKGLGKGGA15K16R17H18R19KVLRDNIQGIT-Am fragment towards Ni(II) was analyzed with multidimensional NMR (1D, 2D TOCSY, NOESY) and UV-Vis spectroscopy. As expected, the coordination involved the imidazolic nitrogen of the His18 residue and the three deprotonated amidic nitrogens of the His18, Arg17 and Lys16 residues, respectively. A model for the structure of the complex was calculated from the inter-residual NOEs recorded in 2D NOESY spectra. The structure obtained shows that the interaction with the metal is responsible for deep changes in the conformation of the peptide, blocking the side chain of Arg17 and Lys16 residues above the coordination plane. These structural modifications may be physiologically relevant to the mechanism of nickel carcinogenesis.

Item Type:Article
ID Code:4811
Status:Published
Refereed:Yes
Uncontrolled Keywords:NMR spectroscopy, Histone H4–Ni(II) interaction, peptide synthesis, nichel
Subjects:Area 03 - Scienze chimiche > CHIM/03 Chimica generale e inorganica
Divisions:001 Università di Sassari > 01 Dipartimenti > Chimica
Publisher:The Royal Society of Chemistry
eISSN:1477-9234
Deposited On:12 Nov 2010 10:42

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