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In vitro oocyte maturation, fertilization and culture after ovum pick-up in an endangered gazelle (Gazella dama mhorr)

Berlinguer, Fiammetta and González, Raquel and Succu, Sara and Del Olmo, Ana and Garde Lopez-Brea, Jose Julian and Espeso Pajares, Gerardo and Gomendio Kindelan, Montserrat and Ledda, Sergio and Roldan, Eduardo R. S. (2008) In vitrooocyte maturation, fertilization and culture after ovum pick-up in an endangered gazelle (Gazella dama mhorr). Theriogenology, Vol. 69 (3), p. 349-359. ISSN 0093-691X. Article.

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DOI: 10.1016/j.theriogenology.2007.10.001

Abstract

The recovery of immature oocytes followed by in vitro maturation, fertilization and culture (IVMFC) allows the rescue of biological material of great genetic value for the establishment of genetic resource banks of endangered species. Studies exist on sperm cryopreservation of endangered Mohor gazelle (Gazella dama mhorr), but no work has been carried out yet on oocyte collection, fertilization and culture in this or related species. The purpose of this study was to develop a protocol for ovarian stimulation for the recovery of oocytes and subsequent IVMFC in the Mohor gazelle using frozen-thawed spermatozoa. Ovum pick-up was performed after ovarian stimulation with a total dose of 5.28 mg of ovine FSH. A total of 35 oocytes were recovered from 56 punctured follicles (62%) (N = 6 females). Out of 29 cumulus-oocyte complexes matured in vitro, 3% were found at germinal vesicle stage, 7% at metaphase I, 21% were degenerated, and 69% advanced to metaphase II. Fertilization and cleavage rates of matured oocytes were 40 and 30%, respectively. Embryos cleaved in vitro up to the 6–8 cell stage but none progressed to the blastocyst stage, suggesting the existence of a developmental block and the need to improve culture conditions. Although more studies are needed to improve hormonal stimulation and oocyte harvesting, as well as IVMFC conditions, this study demonstrates for the first time the feasibility of in vitro fertilization with frozen-thawed semen of in vitro matured oocytes collected by ovum pick-up from FSH-stimulated endangered gazelles.

Item Type:Article
ID Code:4727
Status:Published
Refereed:Yes
Uncontrolled Keywords:Oocyte, sperm, fertilization, embryo, gazelle
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/10 Clinica ostetrica e ginecologia veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Divisions:001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Elsevier
ISSN:0093-691X
Deposited On:22 Oct 2010 16:34

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