Sanna, Maria Paola and Farigu, Serafina and Rocca, Stefano and Pirino, Salvatore and Leoni, Antonio and Sechi, Leonardo Antonio and Lissia, Amelia and Tanda, Francesco and Mingioni, Vindice (2005) Detection of Mycobacterium avium subsp. paratuberculosis (MAP) in animals and human tissue. In: 2. Congresso nazionale A.I.P.Vet.: atti, 20-21 Maggio 2005, Lodi, Italia. Milano, AIPVet. p. 19-22. ISSN 1825-2265. Conference or Workshop Item.
The aim of this work was the detection of MAP in human with CD and in ovine with natural and experimental PTBC, by in “situ” techniques. ISH showed strong signals in the macrophages infiltrating the mucosal and submucosal layers in PTBC samples and positive signals in macrophages of CD cases. Furthermore, ISPCR signals were more numerous than those obtained by ISH. This result indicate that ISPCR is able to detect also less copies of MAP DNA.
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