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Detection of Mycobacterium avium subsp. paratuberculosis (MAP) in animals and human tissue

Sanna, Maria Paola and Farigu, Serafina and Rocca, Stefano and Pirino, Salvatore and Leoni, Antonio and Sechi, Leonardo Antonio and Lissia, Amelia and Tanda, Francesco and Mingioni, Vindice (2005) Detection of Mycobacterium avium subsp. paratuberculosis (MAP) in animals and human tissue. In: 2. Congresso nazionale A.I.P.Vet.: atti, 20-21 Maggio 2005, Lodi, Italia. Milano, AIPVet. p. 19-22. ISSN 1825-2265. Conference or Workshop Item.

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Abstract

The aim of this work was the detection of MAP in human with CD and in ovine with natural and experimental PTBC, by in “situ” techniques. ISH showed strong signals in the macrophages infiltrating the mucosal and submucosal layers in PTBC samples and positive signals in macrophages of CD cases. Furthermore, ISPCR signals were more numerous than those obtained by ISH. This result indicate that ISPCR is able to detect also less copies of MAP DNA.

Item Type:Conference or Workshop Item (Contribute)
ID Code:469
Status:Published
Uncontrolled Keywords:Paratuberculosis, Crohn’s disease, MAP, in situ hybridization (ISH), in situ PCR (ISPCR)
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Area 06 - Scienze mediche > MED/08 Anatomia patologica
Area 07 - Scienze agrarie e veterinarie > VET/03 Patologia generale e anatomia patologica veterinaria
Divisions:002 Altri enti e centri di ricerca del Nord Sardegna > Azienda ASL3, Nuoro > Ospedale "San Francesco" > Servizio di anatomia patologica
001 Università di Sassari > 01 Dipartimenti > Patologia e clinica veterinaria
001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
001 Università di Sassari > 03 Istituti > Anatomia patologica
Publisher:AIPVet
ISSN:1825-2265
Deposited On:18 Aug 2009 10:02

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