titoli, abstracts, parole chiave >>>
Monitoring of Alexandrium species in the Mediterranean Sea through a filter system-PCR assay detection method

Penna, Antonella and Fusco, Giuseppe and Bertozzini, Elena and Giacobbe, Maria Grazia and Vila, Magda and Galluzzi, Luca and Garcés, Esther and Lugliè, Antonella Gesuina Laura and Masó, Mercedes and Magnani, Mauro (2006) Monitoring of Alexandrium species in the Mediterranean Sea through a filter system-PCR assay detection method. African Journal of Marine Science, Vol. 28 (2), p. 241-243. eISSN 1814-2338. Article.

Full text not available from this repository.

DOI: 10.2989/18142320609504155

Alternative URLs:

Abstract

A filter system for collecting phytoplankton cells coupled to a target species-specific polymerase chain reaction (PCR) assay was performed on a spatial and temporal series of net and seawater samples as part of the Mediterranean Sea EU project Strategy (EVK-CT-2001-00046). The application of PCR allowed rapid detection of several harmful dinoflagellate species and genera, including Alexandrium spp. In this study, oligonucleotide primers targeting the 5.8S rDNA and ITS regions of the genus Alexandrium and the species A. minutum were developed. Field samples were concentrated onto filter membranes, total DNA was extracted from mixed phytoplankton populations and PCR assays were carried out with specific primers. Qualitative PCR results were compared with light and epifluorescence microscopic examinations. Results indicated that the molecular assay was able to detect harmful target cells at concentrations undetectable by microscopy. Application of this filter PCR assay to seawater samples revealed it to be a sensitive and rapid procedure for routine monitoring of field samples.

Item Type:Article
ID Code:4630
Status:Published
Refereed:Yes
Uncontrolled Keywords:Alexandrium spp, HAB species, ITS-5.8S rDNA, Mediterranean Sea, PCR
Subjects:Area 05 - Scienze biologiche > BIO/07 Ecologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze botaniche, ecologiche e geologiche
Publisher:Taylor & Francis
eISSN:1814-2338
Deposited On:11 Oct 2010 11:15

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page