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A New automatic system of cell colony counting

Bottigli, Ubaldo and Carpinelli, Massimo and Fiori, Pier Luigi and Golosio, Bruno and Marras, A. and Masala, Giovanni Luca Christian and Oliva, Piernicola (2007) A New automatic system of cell colony counting. International Journal of Mathematical and Computer Sciences, Vol. 3 (4), p. 216-220. ISSN 1305-5313. Article.

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Abstract

The counting process of cell colonies is always a long and laborious process that is dependent on the judgment and ability of the operator. The judgment of the operator in counting can vary in relation to fatigue. Moreover, since this activity is time consuming it can limit the usable number of dishes for each experiment. For these purposes, it is necessary that an automatic system of cell colony counting is used. This article introduces a new automatic system of counting based on the elaboration of the digital images of cellular colonies grown on petri dishes. This system is mainly based on the algorithms of region-growing for the recognition of the regions of interest (ROI) in the image and a Sanger neural net for the characterization of such regions. The better final classification is supplied from a Feed-Forward Neural Net (FF-NN) and confronted with the K-Nearest Neighbour (K-NN) and a Linear Discriminative Function (LDF). The preliminary results are shown.

Item Type:Article
ID Code:4529
Status:Published
Refereed:Yes
Uncontrolled Keywords:Automatic cell counting, neural network, region growing, Sanger net
Subjects:Area 02 - Scienze fisiche > FIS/07 Fisica applicata (a beni culturali, ambientali, biologia e medicina)
Publisher:World Academy of Science, Engineering and Technology
ISSN:1305-5313
Deposited On:13 Sep 2010 09:21

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