titoli, abstracts, parole chiave >>>
The role of afferents to the ventral tegmental area in the handling stress-induced increase in the release of dopamine in the medial prefrontal cortex: a dual-probe microdialysis study in the rat brain

Enrico, Paolo and Bouma, Marjan and De Vries, Jan B. and Westerink, Ben H.C. (1998) The role of afferents to the ventral tegmental area in the handling stress-induced increase in the release of dopamine in the medial prefrontal cortex: a dual-probe microdialysis study in the rat brain. Brain Research, Vol. 779 (1-2), p. 205-213. ISSN 0006-8993. Article.

Full text not available from this repository.

DOI: 10.1016/S0006-8993(97)01132-3

Abstract

This study was aimed to identify the neuronal pathways that mediate the handling stress induced increase in the release of dopamine in the medial prefrontal cortex (mPFC) of the rat brain. For that purpose a microdialysis probe was implanted in the ventral tegmental area (VTA) and a second probe was placed in the ipsilateral mPFC. Receptor specific compounds acting on GABAA (20 μM muscimol), GABAB (50 μM baclofen), acetylcholine (100 μM atropine, 100 μM mecamylamine), NMDA (30, 100 and 300 μM CPP; 300 μM AP-5, 1 mM (+)-HA-966) and non-NMDA receptors (500 μM CNQX) were infused into the VTA by retrograde dialysis, whereas extracellular dopamine was recorded in the ipsilateral mPFC. Intrategmental infusion of muscimol, baclofen, CPP, AP-5, (+)-HA-966 and CNQX decreased extracellular dopamine in the ipsilateral mPFC; atropine and mecamylamine were without effect on the basal values. During infusion of the various compounds rats were gently handled for 15 min. The infusions of muscimol, atropine, mecamylamine and (+)-HA-966 did not modify the handling stress induced increase in extracellular dopamine in the mPFC. However, during intrategmental infusion of baclofen, CPP, AP-5 and CNQX the handling stress induced increase in extracellular dopamine (expressed as % of controls) in the mPFC was suppressed. These results indicate that a glutamatergic projection to the VTA, acting via both NMDA and non-NMDA-glutamate receptors, play a major role in the handling stress-induced increase in dopamine release in the mPFC. In addition the results suggest a certain role for GABAergic neurones, acting via GABAB receptors, in the handling response.

Item Type:Article
ID Code:4367
Status:Published
Refereed:Yes
Uncontrolled Keywords:Dopamine, prefrontal cortex, vta, nmda, stress, microdialysis
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Elsevier
ISSN:0006-8993
Copyright Holders:© 1998 Elsevier Science B.V.
Deposited On:25 Aug 2010 11:50

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page