Zinellu, Angelo and Carru, Ciriaco and Sotgia, Salvatore and Porqueddu, Claudio and Enrico, Paolo and Deiana, Luca (2005) Separation of aceclofenac and diclofenac in human plasma by free zone capillary electrophoresis using N-methyl-d-glucamine as an effective electrolyte additive. European Journal of Pharmaceutical Sciences, Vol. 24 (5), p. 375-380. ISSN 0928-0987. Article.
Full text not available from this repository.
Aceclofenac (A) and diclofenac (D) are effective non-steroidal anti-inflammatory drugs (NSAIDs) derived from the phenylacetic acid with pronounced antirheumatic, anti-inflammatory, analgesic and antipyretic properties. Our work proposes a new, fast-free zone capillary electrophoresis method for the simultaneous determination of aceclofenac and diclofenac in human plasma. The effect of increasing concentrations of N--methyl-d-glucamine organic base on borate run buffer was investigated. A good separation was achieved using a 40 cm × 75 μm uncoated silica capillary, 300 mmol/l sodium borate buffer, 200 mmol/l N--methyl-d-glucamine, pH 8.9, in about 3 min. Moreover, the plasma sample pre-treatment procedure was examined: acidic precipitants such as trichloroacetic acid (TCA), metaphosphoric acid (MPA), perchloric acid (PCA) or 5-sulphosalicylic acid (SSA) cause a total loss of analytes while acetonitrile allows a recovery of 97–98% of both compounds. Its simplicity and rapidity and the low analysis costs demonstrate that our method is a reliable and efficient mean for the comprehensive determination of aceclofenac and diclofenac in human plasma when pharmacokinetics studies are required.
I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore
Repository Staff Only: item control page