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Separation of aceclofenac and diclofenac in human plasma by free zone capillary electrophoresis using N-methyl-d-glucamine as an effective electrolyte additive

Zinellu, Angelo and Carru, Ciriaco and Sotgia, Salvatore and Porqueddu, Claudio and Enrico, Paolo and Deiana, Luca (2005) Separation of aceclofenac and diclofenac in human plasma by free zone capillary electrophoresis using N-methyl-d-glucamine as an effective electrolyte additive. European Journal of Pharmaceutical Sciences, Vol. 24 (5), p. 375-380. ISSN 0928-0987. Article.

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DOI: 10.1016/j.ejps.2004.12.003

Abstract

Aceclofenac (A) and diclofenac (D) are effective non-steroidal anti-inflammatory drugs (NSAIDs) derived from the phenylacetic acid with pronounced antirheumatic, anti-inflammatory, analgesic and antipyretic properties. Our work proposes a new, fast-free zone capillary electrophoresis method for the simultaneous determination of aceclofenac and diclofenac in human plasma. The effect of increasing concentrations of N--methyl-d-glucamine organic base on borate run buffer was investigated. A good separation was achieved using a 40 cm × 75 μm uncoated silica capillary, 300 mmol/l sodium borate buffer, 200 mmol/l N--methyl-d-glucamine, pH 8.9, in about 3 min. Moreover, the plasma sample pre-treatment procedure was examined: acidic precipitants such as trichloroacetic acid (TCA), metaphosphoric acid (MPA), perchloric acid (PCA) or 5-sulphosalicylic acid (SSA) cause a total loss of analytes while acetonitrile allows a recovery of 97–98% of both compounds. Its simplicity and rapidity and the low analysis costs demonstrate that our method is a reliable and efficient mean for the comprehensive determination of aceclofenac and diclofenac in human plasma when pharmacokinetics studies are required.

Item Type:Article
ID Code:4359
Status:Published
Refereed:Yes
Uncontrolled Keywords:Aceclofenac, diclofenac, capillary electrophoresis, acetonitrile
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Elsevier Science
ISSN:0928-0987
Copyright Holders:© 2004 Elsevier B.V. All rights reserved.
Deposited On:25 Aug 2010 11:45

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