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A rapid latex agglutination test for gender identification in the Atlantic bluefin tuna, Thunnus thynnus (Linnaeus)

Micera, Elisabetta and Zupa, Rosa and Zarrilli, Antonia and Camarda, Antonio and Moramarco, Angela M. and Acone, Franca and De Metrio, Gregorio and Corriero, Aldo (2010) A rapid latex agglutination test for gender identification in the Atlantic bluefin tuna, Thunnus thynnus (Linnaeus). Aquaculture Research, Vol. 41 (9), p. 1396-1401. eISSN 1365-2109. Article.

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DOI: 10.1111/j.1365-2109.2009.02429.x

Abstract

A rapid, one-step agglutination assay has been developed, based on latex particles sensitized with antibodies against vitellogenin (Vtg), aimed at Atlantic bluefin tuna, Thunnus thynnus (Linnaeus) (ABFT), gender identification. The egg-yolk precursor protein Vtg was used as a gender marker for the assay as it is a female-specific protein synthesized during reproductive maturation. The presence of Vtg in the plasma was revealed in 60-120 s through an agglutination reaction by mixing small volumes of ABFT plasma and an anti-Vtg antibody-latex suspension on a microscope slide. The effectiveness of the present test was restricted to the months of May and June, concomitant with high circulating Vtg levels. Because of its rapidity and ease of performance in the field, the present gender identification assay could be useful for broodstock management in the aquaculture industry as well as in tagging studies on wild populations.

Item Type:Article
ID Code:4340
Status:Published
Refereed:Yes
Uncontrolled Keywords:Bluefin tuna, sex determination, vitellogenin, latex agglutination
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/01 Anatomia degli animali domestici
Divisions:001 Università di Sassari > 01 Dipartimenti > Biologia animale
Publisher:Wiley
eISSN:1365-2109
Copyright Holders:© 2009 The Authors, Journal Compilation © 2009 Blackwell Publishing Ltd
Deposited On:24 Aug 2010 14:29

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