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Analysis of loss of heterozygosity in neoplastic nodules induced by diethylnitrosomine in the resistant BFF1 rat strain.

Gariboldi, Manuela and Pascale, Rosa Maria and Manenti, Giacomo and De Miglio, Maria Rosaria and Calvisi, Diego Francesco and Carru, Angelo and Dragani, Tommaso A. and Feo, Francesco (1999) Analysis of loss of heterozygosity in neoplastic nodules induced by diethylnitrosomine in the resistant BFF1 rat strain. Carcinogenesis, Vol. 20 (7), p. 1363-1368. ISSN 1460-2180. Article.

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DOI: 10.1093/carcin/20.7.1363


Loss of heterozygosity (LOH) at specific chromosomal regions is a frequent event in poorly differentiated human hepatocellular carcinomas (HCCs), but rare in mouse HCCs. This behavior could depend on interspecies differences in mechanisms of hepatocarcinogenesis or in developmental stage of lesions. To verify if LOH is involved in rat hepatocarcinogenesis, we studied LOH frequency in slowly growing neoplastic nodules induced by Solt–Farber model in diethylnitrosamine-initiated BFF1 rats. We analyzed, with microsatellites, markers at 67 rat loci dispersed over all chromosomes, corresponding to regions homologous to those lost in human HCCs or containing hepatocellular susceptibility (Hcs) or resistance (Hcr) loci in rat and mouse. In agreement with previous findings with mouse HCCs, but at variance with human HCCs, no detectable LOH was found at any locus in rats, suggesting rare LOH involvement in neoplastic nodules, with low tendency to progress to full malignancy, of BFF1 rats.

Item Type:Article
ID Code:4327
Uncontrolled Keywords:2-Acetylaminofluorene, animals, carcinoma hepatocellular, chromosome mapping, diethylnitrosamine, genetic markers, liver, liver neoplasms, loss of heterozygosity, male, microsatellite repeats, polymerase chain reaction, polymorphism, single-stranded conformational, rats, rats inbred bn, rats inbred f344
Subjects:Area 06 - Scienze mediche > MED/04 Patologia generale
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Oxford University Press
Copyright Holders:© University Press
Deposited On:23 Aug 2010 13:39

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