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HLA-DQB1*0305 and −DQB1*0304 alleles among Sardinians: evolutionary and practical implications for oligotyping

Cucca, Francesco and Frau, Fulvia and Lampis, Rosanna and Floris, Monica and Argiolas, Luisa and Macis, Maria Doloretta and Cao, Antonio and De Virgiliis, Stefano and Congia, Mauro (1994) HLA-DQB1*0305 and −DQB1*0304 alleles among Sardinians: evolutionary and practical implications for oligotyping. Human Immunology, Vol. 40 (2), p. 143-149. ISSN 0198-8859. Article.

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DOI: 10.1016/0198-8859(94)90060-4

Abstract

This study, performed in individuals of Sardiaian descent, reports an epidemiologic and molecular analysis of the recently identified DQB1*0304 and DQB1*0305 alleles*. These two alleles having a gene frequency of 0.017 and 0.005, respectively, are not uncommon in Sardinia and are distributed fairly uniformly on the island. The analysis of DQB1 second and third exons of the two alleles revealed that although they have always been found included within the same DRB1*0403-DQA1*03 haplotype, they had a different origin. The sequence pattern of DQB1*0305 confirmed that it originated from the DQB1*0302 “recipient” gene by the insertion of a DQB1*0402 nucleotide stretch, within its β-sheet region, while that of DQB1*0304 suggested that it originated from the DQB1*0301 gene, either by a single point mutation at codon 57 (GCC instead of GAC) or, alternatively, by a segmental transfer of a DQB1*0302 motif, including codon 57, within its α-helic region. Independently from the mechanism of generation, the fact that DQB1*0304 originated from DQB1*0301 allele was intriguing considering that, in over 1500 HLA class II Sardinian haplotypes examined, neither the putative parental DRB1*0403-DQA1*03-DQB1*0301 nor any DRB1*04-DQA1*03-DQB1*0301 haplotypes were found. Finally, since the assignment of DQB1*0305 may be inaccurate with the traditional panel of probes commonly used for DQB1 oligotyping, the use of an additional oligonucleotide probe is recommended.

Item Type:Article
ID Code:4272
Status:Published
Refereed:Yes
Uncontrolled Keywords:Dependent diabetes-mellitus, major histocompatibility complex, polymerase chain-reaction, humania antigens, dr-beta loci, gene conversion, oligonucleotide probes, extended haplotype, HLA, susceptibility, Sardinia
Subjects:Area 06 - Scienze mediche > MED/03 Genetica medica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Elsevier Science
ISSN:0198-8859
Deposited On:26 Jul 2010 16:07

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