Sanna, Maria Giovanna and Mangia, Nicoletta Pasqualina and Garau, Giovanni and Murgia, Marco Ambrogio and Massa, Tomasina G. and Franco, Mario Andrea and Deiana, Pietrino (2005) Selection of folate-producing lactic acid bacteria for improving fermented goat milk. Italian Journal of Food Science, Vol. 17 (2), p. 143-154. ISSN 1120-1770. Article.
Goat milk is a complete food but its low level of folic acid diminisches its nutritional efficacy. In this study, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lb. delbrueckii subsp. lactis and Lb. helveticus strains were selected for folate production in goat milk to improve its quality. A suitable HPLC method was developed to directly determine both total folate and its biologically active derivatives such as 5-methyl-tetrahydrofolate (5- CH3-H4-PteGlu), tetrahydrofolate (H4-PteGlu) and 5 formyl-tetrahydrofolate (5-CHO-H4-PteGlu). Results showed that S. thermophilus, Lb. delbrueckii subsp. lactis and Lb. helveticus strains produced higher amounts of total folate than Lb. delbrueckii subsp. bulgaricus, especially 5-CH3-H4-PteGlu and H4-PteGlu. Moreover the use of selected S. thermophilus strains in association with Lb. delbrueckii subsp. bulgaricus resulted in yoghurt with a significant quantity of folate and good organoleptic features.
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