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Combinations of specific DRB1, DQA1, DQB1 haplotypes are associated with insulin-dependent diabetes mellitus in Sardinia

Cucca, Francesco and Muntoni, Francesco M. and Lampis, Rosanna and Frau, Fulvia and Argiolas, Luisa and Silvetti, Mario and Angius, Efisio and Cao, Antonio and De Virgiliis, Stefano and Congia, Mauro (1993) Combinations of specific DRB1, DQA1, DQB1 haplotypes are associated with insulin-dependent diabetes mellitus in Sardinia. Human Immunology, Vol. 37 (2), p. 85-94. ISSN 0198-8859. Article.

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DOI: 10.1016/0198-8859(93)90146-R

Abstract

The Sardinian population has an extremely high incidence of IDDM (30.2 of 100.000 in the age group of 0–14 years). This study reports the molecular characterization of HLA class II genes in 120 IDDM sporadic patients and 89 healthy subjects of Sardinian origin. Compared with other Caucasians, both Sardinian patients and controls had an unusual distribution of haplotypes and genotypes. In particular, there was a high gene frequency of the DRB1*0301, DQA1*0501, DQB1*0201 susceptibility haplotype both in patients (0.58) and controls (0.23) while a reduction of the BRB1*1501, DQA1*0102, DQB1*0602 protective haplotype (0.03) was observed in the healthy population. This distribution may partially explain the high incidence of IDDM reported in Sardinia. The analysis of the DQβ57 and DQα52 residues showed that the absence of Asp 57 and the presence of Arg 52 were associated with IDDM in a dose-response manner. On the other hand, we found that (a) a very similar distribution of these residues was found when comparing Sardinians with another healthy Caucasian population from the same latitude but with a lower rate of IDDM incidence; (b) several genotypes encoding the identical DQα52/DQβ57 phenotype carried very differnt relative risks; and (c) the DRB1*0403, DQA1*0301, DQB1*0304 haplotype (DQβ57 Asp-neg and DQα52 Argpos) was found in 40% of the DR4-positive controls but not in patients (p = 0.00034), while the DRB1*0405, DQA1*0301, and DQB1*0302 haplotype carrying the same residues at the same positions was found in 70% of the DR4-positive patients and in only one control (p = 0.00003). These findings suggest that S0IDDM susceptibility cannot be completely explained by the model in which only DQα52 and DQβ57 residues are taken into account.

Item Type:Article
ID Code:4251
Status:Published
Refereed:Yes
Uncontrolled Keywords:Polymerase chain-reaction, celiac-disease patients, amino-acid, HLA-DR, confers susceptibility, oligonucleotide probes, extended haplotype, high-frequency, beta-chain, DNA
Subjects:Area 06 - Scienze mediche > MED/03 Genetica medica
Divisions:001 Università di Sassari > 01 Dipartimenti > Scienze biomediche
Publisher:Elsevier
ISSN:0198-8859
Deposited On:23 Jul 2010 12:30

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